Glutamine administration modulates lung γδ T lymphocyte expression in mice with polymicrobial sepsis

Ya Mei Hu, Chiu Li Yeh, Man Hui Pai, Wan Yun Lee, Sung Ling Yeh

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

ABSTRACT: This study investigated the effects of glutamine (GLN) administration on regulating lung γδ T cells in polymicrobial sepsis. Mice were randomly assigned to normal group (NC), septic saline group (SS), and septic GLN group (SG). All mice were fed with chow diet. Sepsis was induced by cecal ligation and puncture (CLP). The SS and SG groups were, respectively, injected with saline and 0.75 g GLN/kg body weight once via tail vein 1 h after CLP. Mice were killed 12 and 24 h after CLP. Their lungs were collected for further analysis. The results showed that, compared with normal mice, sepsis resulted in higher lung γδ T cell and neutrophil percentages and higher cytokine expressed by γδ T cells. Histopathologic findings showed that the extent of inflammatory lesions of the lung alveolar was less severe in the SG group than the SS group after CLP. The SG group had a higher γδ T cell percentage and lower γδ T cell apoptotic rates as well as lower neutrophil numbers in the lungs. Also, interleukin 17A (IL-17A), interferon γ, and IL-10 expressed by γδ T cells and CXC receptor 2 expressed by neutrophils decreased in the SG group. Moreover, GLN reduced IL-17A, IL-1β, and IL-23 concentrations and myeloperoxidase activity in lung tissues. Our results suggest that GLN administration after initiation of sepsis affects lung γδ T cell percentage and cytokine secretion and prevented apoptosis of γδ T cells and neutrophil infiltration to the lungs, which may partly be responsible for ameliorating acute lung injury induced by sepsis.

Original languageEnglish
Pages (from-to)115-122
Number of pages8
JournalShock
Volume41
Issue number2
DOIs
Publication statusPublished - Feb 2014

Fingerprint

Glutamine
Sepsis
T-Lymphocytes
Lung
Punctures
Ligation
Neutrophils
Interleukin-17
Cytokines
Interleukin-23
Neutrophil Infiltration
Acute Lung Injury
T-Cell Antigen Receptor
Interleukin-1
Interleukin-10
Interferons
Peroxidase
Tail
Veins
Body Weight

Keywords

  • Acute lung injury
  • apoptosis
  • CXC chemokine receptor 2
  • interleukin 17
  • neutrophil

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

Cite this

Glutamine administration modulates lung γδ T lymphocyte expression in mice with polymicrobial sepsis. / Hu, Ya Mei; Yeh, Chiu Li; Pai, Man Hui; Lee, Wan Yun; Yeh, Sung Ling.

In: Shock, Vol. 41, No. 2, 02.2014, p. 115-122.

Research output: Contribution to journalArticle

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