Glutamine Administration Modulates Endothelial Progenitor Cell and Lung Injury in Septic Mice

Man Hui Pai, Yao Ming Shih, Juey Ming Shih, Chiu Li Yeh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

ABSTRACT: This study investigated the effects of glutamine (GLN) administration on circulating endothelial progenitor cells (EPCs) and lung angiopoietin (Ang) gene expressions in polymicrobial sepsis. Mice were randomly assigned to a normal control group (NC), septic saline group (SS), and septic GLN group (SG). All mice were fed with a chow diet. Sepsis was induced by cecal ligation and puncture (CLP). The mice in SS group were injected with saline whereas SG group administered 0.75?g GLN/kg body weight once via tail vein 1?h after CLP. Mice were sacrificed 24, and 48?h post-CLP. Their blood and lungs were collected for further analysis. The results showed that, compared to normal mice, sepsis resulted in higher C-X-C motif chemokine-12, vascular endothelial growth factor, nitric oxide levels and a higher circulating EPC percentage. Also, inflammatory cytokine concentrations and Ang-2 gene expression were upregulated in lung tissues. GLN administration enhanced the mobilization of EPC, downregulated inflammatory cytokine production and the Ang-2 gene expressions in lungs. Histopathological findings showed that, the extent of inflammatory lesions of the lung alveolar was less severe in the SG group than the SS group after CLP. Our results suggest that a single dose of intravenous GLN administration after initiation of sepsis promote the mobilization of circulating EPC and modulates the balance of Ang-Tie2 axis that may improve the vascular function, ameliorate inflammation and protect lung injury against polymicrobial sepsis.

Original languageEnglish
Pages (from-to)587-592
JournalShock
Volume46
Issue number5
DOIs
Publication statusPublished - Oct 1 2016

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Lung Injury
Glutamine
Sepsis
Punctures
Ligation
Lung
Angiopoietins
Angiopoietin-2
Gene Expression
Cytokines
CXC Chemokines
Intravenous Administration
Vascular Endothelial Growth Factor A
Blood Vessels
Tail
Veins
Nitric Oxide
Down-Regulation
Body Weight
Endothelial Progenitor Cells

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

Cite this

Glutamine Administration Modulates Endothelial Progenitor Cell and Lung Injury in Septic Mice. / Pai, Man Hui; Shih, Yao Ming; Shih, Juey Ming; Yeh, Chiu Li.

In: Shock, Vol. 46, No. 5, 01.10.2016, p. 587-592.

Research output: Contribution to journalArticle

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