Glutamate-mediated spinal reflex potentiation involves ERK 1/2 phosphorylation in anesthetized rats

Hsien Yu Peng, Ya Wen Cheng, Shin Da Lee, Yen Chun Ho, Dylan Chou, Gin Den Chen, Chen Li Cheng, Tien Huan Hsu, Kwong Chung Tung, Tzer Bin Lin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The extracellular signal-regulated kinase (ERK) cascades are suggested to contribute to excitatory plasticity in the CNS, including the spinal cord. This study investigated whether the ERK involves in the repetitive stimulation-induced spinal reflex potentiation (SRP) in the pelvic nerve-to-external urethra sphincter reflex activities. External urethra sphincter electromyogram in response to pelvic afferent nerve test stimulation (TS, 1/30 Hz) or repetitive stimulation (RS, 1 Hz) was recorded in anesthetized rats. TS evoked a baseline reflex activity, whereas RS produced SRP in associated with significant ERK 1/2 phosphorylation. RS-induced SRP and ERK 1/2 phosphorylation were both abolished by pretreatment of U0126 (MEK inhibitor). Intrathecal CNQX (AMPA receptor antagonist) attenuated, while AP5 (NMDA receptor antagonist) abolished the RS-induced SRP and ERK 1/2 phosphorylation. Pretreated U0126 abolished the SRP elicited by glutamatergic agonists including glutamate, NMDA and AMPA. Intrathecal H89 and BIS7 (PKA and PKC inhibitors, respectively) both abolished the RS- and glutamate agonist-induced SRP as well as ERK 1/2 phosphorylation. In addition, forskolin and PMA (PKA and PKC activator, respectively) induced SRP, which were both abolished by pretreated U0126. Saline distension, mimicking the storage phase of the urinary bladder, induced SRP and ERK 1/2 phosphorylation. In conclusion, activated ERK 1/2 may produce SRP in the pelvic nerve-to-external urethra sphincter reflex activity, which is essential for urine continence. In addition, blockage of spinal ERK 1/2 activation decreases the physiological function of the urethra, indicating that phosphorylation of the ERK 1/2 cascade may represent a novel target for the treatment of patients with neurological incontinence of spinal origin.

Original languageEnglish
Pages (from-to)686-698
Number of pages13
JournalNeuropharmacology
Volume54
Issue number4
DOIs
Publication statusPublished - Mar 2008
Externally publishedYes

Fingerprint

Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Reflex
Glutamic Acid
Phosphorylation
Urethra
Excitatory Amino Acid Agonists
Extracellular Signal-Regulated MAP Kinases
6-Cyano-7-nitroquinoxaline-2,3-dione
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
AMPA Receptors
Mitogen-Activated Protein Kinase Kinases
Electromyography
Colforsin
N-Methylaspartate
N-Methyl-D-Aspartate Receptors
Spinal Cord
Urinary Bladder
Urine

Keywords

  • Extracellular signal-regulated kinase
  • Glutamate
  • Phosphorylation
  • Protein kinase A
  • Protein kinase C

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

Glutamate-mediated spinal reflex potentiation involves ERK 1/2 phosphorylation in anesthetized rats. / Peng, Hsien Yu; Cheng, Ya Wen; Lee, Shin Da; Ho, Yen Chun; Chou, Dylan; Chen, Gin Den; Cheng, Chen Li; Hsu, Tien Huan; Tung, Kwong Chung; Lin, Tzer Bin.

In: Neuropharmacology, Vol. 54, No. 4, 03.2008, p. 686-698.

Research output: Contribution to journalArticle

Peng, HY, Cheng, YW, Lee, SD, Ho, YC, Chou, D, Chen, GD, Cheng, CL, Hsu, TH, Tung, KC & Lin, TB 2008, 'Glutamate-mediated spinal reflex potentiation involves ERK 1/2 phosphorylation in anesthetized rats', Neuropharmacology, vol. 54, no. 4, pp. 686-698. https://doi.org/10.1016/j.neuropharm.2007.11.017
Peng, Hsien Yu ; Cheng, Ya Wen ; Lee, Shin Da ; Ho, Yen Chun ; Chou, Dylan ; Chen, Gin Den ; Cheng, Chen Li ; Hsu, Tien Huan ; Tung, Kwong Chung ; Lin, Tzer Bin. / Glutamate-mediated spinal reflex potentiation involves ERK 1/2 phosphorylation in anesthetized rats. In: Neuropharmacology. 2008 ; Vol. 54, No. 4. pp. 686-698.
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