Glucose-regulated protein 78 mediates hormone-independent prostate cancer progression and metastasis through maspin and COX-2 expression

Chun Te Wu, Wen Ching Wang, Miao Fen Chen, Hou Yu Su, Wei Yu Chen, Chih Hsiung Wu, Yu Jia Chang, Hui Hsiung Liu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Glucose-regulated protein 78 (GRP78) plays an essential role in embryonic development and in the progression and therapeutic resistance of many cancers. However, little is known about the function of GRP78 in hormone-independent prostate cancer. Here, we found that the expression levels of GRP78 were higher in PC-3 cells than in DU-145 cells. When the expression of GRP78 was silenced using a GRP78-specific small interfering RNA in PC-3 cells, the growth rate and adhesive ability were reduced. Cell migration was dramatically decreased in GRP78-depleted cells. Dissection of the involved signal pathways revealed that maspin expression was upregulated after silencing GRP78 expression. The upregulation of maspin and downregulation of COX-2 may cause the decrease in cell proliferation and migration observed after silencing GRP78 expression. Silencing GRP78 expression may suppress the proliferation, adhesion, and migration of prostate cancer cells via maspin and COX-2 regulation.

Original languageEnglish
Pages (from-to)195-204
Number of pages10
JournalTumor Biology
Volume35
Issue number1
DOIs
Publication statusPublished - Jan 2014

Fingerprint

Prostatic Neoplasms
Hormones
Neoplasm Metastasis
Cell Movement
SERPIN-B5
glucose-regulated proteins
Adhesives
Small Interfering RNA
Embryonic Development
Dissection
Signal Transduction
Up-Regulation
Down-Regulation
Cell Proliferation
Growth
Neoplasms

Keywords

  • COX-2
  • GRP78
  • Maspin
  • Migration
  • Prostate cancer

ASJC Scopus subject areas

  • Cancer Research

Cite this

Glucose-regulated protein 78 mediates hormone-independent prostate cancer progression and metastasis through maspin and COX-2 expression. / Wu, Chun Te; Wang, Wen Ching; Chen, Miao Fen; Su, Hou Yu; Chen, Wei Yu; Wu, Chih Hsiung; Chang, Yu Jia; Liu, Hui Hsiung.

In: Tumor Biology, Vol. 35, No. 1, 01.2014, p. 195-204.

Research output: Contribution to journalArticle

Wu, Chun Te ; Wang, Wen Ching ; Chen, Miao Fen ; Su, Hou Yu ; Chen, Wei Yu ; Wu, Chih Hsiung ; Chang, Yu Jia ; Liu, Hui Hsiung. / Glucose-regulated protein 78 mediates hormone-independent prostate cancer progression and metastasis through maspin and COX-2 expression. In: Tumor Biology. 2014 ; Vol. 35, No. 1. pp. 195-204.
@article{bd781a8961c244dd9cd82aa1be81f4de,
title = "Glucose-regulated protein 78 mediates hormone-independent prostate cancer progression and metastasis through maspin and COX-2 expression",
abstract = "Glucose-regulated protein 78 (GRP78) plays an essential role in embryonic development and in the progression and therapeutic resistance of many cancers. However, little is known about the function of GRP78 in hormone-independent prostate cancer. Here, we found that the expression levels of GRP78 were higher in PC-3 cells than in DU-145 cells. When the expression of GRP78 was silenced using a GRP78-specific small interfering RNA in PC-3 cells, the growth rate and adhesive ability were reduced. Cell migration was dramatically decreased in GRP78-depleted cells. Dissection of the involved signal pathways revealed that maspin expression was upregulated after silencing GRP78 expression. The upregulation of maspin and downregulation of COX-2 may cause the decrease in cell proliferation and migration observed after silencing GRP78 expression. Silencing GRP78 expression may suppress the proliferation, adhesion, and migration of prostate cancer cells via maspin and COX-2 regulation.",
keywords = "COX-2, GRP78, Maspin, Migration, Prostate cancer",
author = "Wu, {Chun Te} and Wang, {Wen Ching} and Chen, {Miao Fen} and Su, {Hou Yu} and Chen, {Wei Yu} and Wu, {Chih Hsiung} and Chang, {Yu Jia} and Liu, {Hui Hsiung}",
year = "2014",
month = "1",
doi = "10.1007/s13277-013-1024-4",
language = "English",
volume = "35",
pages = "195--204",
journal = "Tumor Biology",
issn = "1010-4283",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Glucose-regulated protein 78 mediates hormone-independent prostate cancer progression and metastasis through maspin and COX-2 expression

AU - Wu, Chun Te

AU - Wang, Wen Ching

AU - Chen, Miao Fen

AU - Su, Hou Yu

AU - Chen, Wei Yu

AU - Wu, Chih Hsiung

AU - Chang, Yu Jia

AU - Liu, Hui Hsiung

PY - 2014/1

Y1 - 2014/1

N2 - Glucose-regulated protein 78 (GRP78) plays an essential role in embryonic development and in the progression and therapeutic resistance of many cancers. However, little is known about the function of GRP78 in hormone-independent prostate cancer. Here, we found that the expression levels of GRP78 were higher in PC-3 cells than in DU-145 cells. When the expression of GRP78 was silenced using a GRP78-specific small interfering RNA in PC-3 cells, the growth rate and adhesive ability were reduced. Cell migration was dramatically decreased in GRP78-depleted cells. Dissection of the involved signal pathways revealed that maspin expression was upregulated after silencing GRP78 expression. The upregulation of maspin and downregulation of COX-2 may cause the decrease in cell proliferation and migration observed after silencing GRP78 expression. Silencing GRP78 expression may suppress the proliferation, adhesion, and migration of prostate cancer cells via maspin and COX-2 regulation.

AB - Glucose-regulated protein 78 (GRP78) plays an essential role in embryonic development and in the progression and therapeutic resistance of many cancers. However, little is known about the function of GRP78 in hormone-independent prostate cancer. Here, we found that the expression levels of GRP78 were higher in PC-3 cells than in DU-145 cells. When the expression of GRP78 was silenced using a GRP78-specific small interfering RNA in PC-3 cells, the growth rate and adhesive ability were reduced. Cell migration was dramatically decreased in GRP78-depleted cells. Dissection of the involved signal pathways revealed that maspin expression was upregulated after silencing GRP78 expression. The upregulation of maspin and downregulation of COX-2 may cause the decrease in cell proliferation and migration observed after silencing GRP78 expression. Silencing GRP78 expression may suppress the proliferation, adhesion, and migration of prostate cancer cells via maspin and COX-2 regulation.

KW - COX-2

KW - GRP78

KW - Maspin

KW - Migration

KW - Prostate cancer

UR - http://www.scopus.com/inward/record.url?scp=84893717009&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893717009&partnerID=8YFLogxK

U2 - 10.1007/s13277-013-1024-4

DO - 10.1007/s13277-013-1024-4

M3 - Article

C2 - 23922175

AN - SCOPUS:84893717009

VL - 35

SP - 195

EP - 204

JO - Tumor Biology

JF - Tumor Biology

SN - 1010-4283

IS - 1

ER -