Glucose-Regulated protein 78 (GRP78) silencing enhances cell migration but does not influence cell proliferation in hepatocellular carcinoma

Yu-Jia Chang, Chong Chi Chiu, Chih-Hsiung Wu, Jane An, Cheng Chia Wu, Tsan Zon Liu, Po-Li Wei, Ming-Te Huang

Research output: Contribution to journalArticle

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Abstract

Background. GRP78 plays an essential role in embryonic development and in the therapeutic treatment and progression of cancer. However, little is known about the role of GRP78 in hepatocellular carcinoma (HCC). Methods. In this study, we characterized five different HCC cell lines to examine GRP78 expression patterns and found that only HepJ5 cells ectopically overexpress GRP78. We knocked down GRP78 expression in HepJ5 cells using a small interfering RNA (siRNA), and the proliferation assay and migration assay were performed. Results. Using siRNA technique, we could successfully reduce GRP78 expression levels in HepJ5 cells. In a cell growth study, we found that GRP78-siRNA caused no significant changes in cellular proliferation in 3-(4, 5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation, and cell cycle distribution. In a cell migration study, we found that GRP78-siRNA HepJ5 cells had dramatically increased migration ability in Transwell assay. Conclusions. We conclude that ectopically expressed GRP78 does not contribute to the increased proliferation of HepJ5 cells, but does correlate with the migration of HCC cells under normoxic conditions.

Original languageEnglish
Pages (from-to)1703-1709
Number of pages7
JournalAnnals of Surgical Oncology
Volume17
Issue number6
DOIs
Publication statusPublished - Jun 2010

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Cell Movement
Hepatocellular Carcinoma
Cell Proliferation
Small Interfering RNA
glucose-regulated proteins
Embryonic Development
Cell Cycle
Cell Line
Growth
Neoplasms

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

Glucose-Regulated protein 78 (GRP78) silencing enhances cell migration but does not influence cell proliferation in hepatocellular carcinoma. / Chang, Yu-Jia; Chiu, Chong Chi; Wu, Chih-Hsiung; An, Jane; Wu, Cheng Chia; Liu, Tsan Zon; Wei, Po-Li; Huang, Ming-Te.

In: Annals of Surgical Oncology, Vol. 17, No. 6, 06.2010, p. 1703-1709.

Research output: Contribution to journalArticle

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abstract = "Background. GRP78 plays an essential role in embryonic development and in the therapeutic treatment and progression of cancer. However, little is known about the role of GRP78 in hepatocellular carcinoma (HCC). Methods. In this study, we characterized five different HCC cell lines to examine GRP78 expression patterns and found that only HepJ5 cells ectopically overexpress GRP78. We knocked down GRP78 expression in HepJ5 cells using a small interfering RNA (siRNA), and the proliferation assay and migration assay were performed. Results. Using siRNA technique, we could successfully reduce GRP78 expression levels in HepJ5 cells. In a cell growth study, we found that GRP78-siRNA caused no significant changes in cellular proliferation in 3-(4, 5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation, and cell cycle distribution. In a cell migration study, we found that GRP78-siRNA HepJ5 cells had dramatically increased migration ability in Transwell assay. Conclusions. We conclude that ectopically expressed GRP78 does not contribute to the increased proliferation of HepJ5 cells, but does correlate with the migration of HCC cells under normoxic conditions.",
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AU - Chang, Yu-Jia

AU - Chiu, Chong Chi

AU - Wu, Chih-Hsiung

AU - An, Jane

AU - Wu, Cheng Chia

AU - Liu, Tsan Zon

AU - Wei, Po-Li

AU - Huang, Ming-Te

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AB - Background. GRP78 plays an essential role in embryonic development and in the therapeutic treatment and progression of cancer. However, little is known about the role of GRP78 in hepatocellular carcinoma (HCC). Methods. In this study, we characterized five different HCC cell lines to examine GRP78 expression patterns and found that only HepJ5 cells ectopically overexpress GRP78. We knocked down GRP78 expression in HepJ5 cells using a small interfering RNA (siRNA), and the proliferation assay and migration assay were performed. Results. Using siRNA technique, we could successfully reduce GRP78 expression levels in HepJ5 cells. In a cell growth study, we found that GRP78-siRNA caused no significant changes in cellular proliferation in 3-(4, 5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation, and cell cycle distribution. In a cell migration study, we found that GRP78-siRNA HepJ5 cells had dramatically increased migration ability in Transwell assay. Conclusions. We conclude that ectopically expressed GRP78 does not contribute to the increased proliferation of HepJ5 cells, but does correlate with the migration of HCC cells under normoxic conditions.

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