Abstract

Background: Glucose-regulated protein 78 (GRP78) plays an important role in the therapeutic treatment and progression of cancer. However, little is known about the effect of GRP78 expression to curcumin in hepatocellular carcinoma (HCC). Materials and Methods: In this study, we generated GRP78 knockdown cells (GRP78KD) by a short interfering RNA (siRNA) technique. The antiproliferation effects of curcumin were determined by MTT assay, TUNEL assay, and cell cycle determination. Results: We found that GRP78KD cells were more resistant to curcumin treatment compared with the parental cells in MTT assay. The apoptosis cell population was increased in scrambled-siRNA cells treated with curcumin compared with GRP78KD cells in cell cycle distribution and TUNEL assays. Finally, we found that knocking down GRP78 causes resistance to curcumin treatment through the suppression of caspase-3 and caspase-8 expression levels. Conclusions: We conclude that the expression level of GRP78 may contribute to the therapeutic effect of curcumin on HCC cells.

Original languageEnglish
Pages (from-to)2395-2403
Number of pages9
JournalAnnals of Surgical Oncology
Volume18
Issue number8
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Curcumin
Hepatocellular Carcinoma
In Situ Nick-End Labeling
Small Interfering RNA
Cell Cycle
glucose-regulated proteins
Caspase 8
Therapeutic Uses
Caspase 3
Apoptosis

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

@article{012d42bb75324dbab2a7f756ba844ced,
title = "Glucose-regulated protein 78 (GRP78) mediated the efficacy to curcumin treatment on hepatocellular carcinoma",
abstract = "Background: Glucose-regulated protein 78 (GRP78) plays an important role in the therapeutic treatment and progression of cancer. However, little is known about the effect of GRP78 expression to curcumin in hepatocellular carcinoma (HCC). Materials and Methods: In this study, we generated GRP78 knockdown cells (GRP78KD) by a short interfering RNA (siRNA) technique. The antiproliferation effects of curcumin were determined by MTT assay, TUNEL assay, and cell cycle determination. Results: We found that GRP78KD cells were more resistant to curcumin treatment compared with the parental cells in MTT assay. The apoptosis cell population was increased in scrambled-siRNA cells treated with curcumin compared with GRP78KD cells in cell cycle distribution and TUNEL assays. Finally, we found that knocking down GRP78 causes resistance to curcumin treatment through the suppression of caspase-3 and caspase-8 expression levels. Conclusions: We conclude that the expression level of GRP78 may contribute to the therapeutic effect of curcumin on HCC cells.",
author = "Chang, {Yu Jia} and Tai, {Cheng Jeng} and Kuo, {Li Jen} and Wei, {Po Li} and Liang, {Hung Hua} and Liu, {Tsan Zon} and Weu Wang and Tai, {Chen Jei} and Ho, {Yuan Soon} and Wu, {Chih Hsiung} and Huang, {Ming Te}",
year = "2011",
month = "8",
doi = "10.1245/s10434-011-1597-3",
language = "English",
volume = "18",
pages = "2395--2403",
journal = "Annals of Surgical Oncology",
issn = "1068-9265",
publisher = "Springer New York",
number = "8",

}

TY - JOUR

T1 - Glucose-regulated protein 78 (GRP78) mediated the efficacy to curcumin treatment on hepatocellular carcinoma

AU - Chang, Yu Jia

AU - Tai, Cheng Jeng

AU - Kuo, Li Jen

AU - Wei, Po Li

AU - Liang, Hung Hua

AU - Liu, Tsan Zon

AU - Wang, Weu

AU - Tai, Chen Jei

AU - Ho, Yuan Soon

AU - Wu, Chih Hsiung

AU - Huang, Ming Te

PY - 2011/8

Y1 - 2011/8

N2 - Background: Glucose-regulated protein 78 (GRP78) plays an important role in the therapeutic treatment and progression of cancer. However, little is known about the effect of GRP78 expression to curcumin in hepatocellular carcinoma (HCC). Materials and Methods: In this study, we generated GRP78 knockdown cells (GRP78KD) by a short interfering RNA (siRNA) technique. The antiproliferation effects of curcumin were determined by MTT assay, TUNEL assay, and cell cycle determination. Results: We found that GRP78KD cells were more resistant to curcumin treatment compared with the parental cells in MTT assay. The apoptosis cell population was increased in scrambled-siRNA cells treated with curcumin compared with GRP78KD cells in cell cycle distribution and TUNEL assays. Finally, we found that knocking down GRP78 causes resistance to curcumin treatment through the suppression of caspase-3 and caspase-8 expression levels. Conclusions: We conclude that the expression level of GRP78 may contribute to the therapeutic effect of curcumin on HCC cells.

AB - Background: Glucose-regulated protein 78 (GRP78) plays an important role in the therapeutic treatment and progression of cancer. However, little is known about the effect of GRP78 expression to curcumin in hepatocellular carcinoma (HCC). Materials and Methods: In this study, we generated GRP78 knockdown cells (GRP78KD) by a short interfering RNA (siRNA) technique. The antiproliferation effects of curcumin were determined by MTT assay, TUNEL assay, and cell cycle determination. Results: We found that GRP78KD cells were more resistant to curcumin treatment compared with the parental cells in MTT assay. The apoptosis cell population was increased in scrambled-siRNA cells treated with curcumin compared with GRP78KD cells in cell cycle distribution and TUNEL assays. Finally, we found that knocking down GRP78 causes resistance to curcumin treatment through the suppression of caspase-3 and caspase-8 expression levels. Conclusions: We conclude that the expression level of GRP78 may contribute to the therapeutic effect of curcumin on HCC cells.

UR - http://www.scopus.com/inward/record.url?scp=80051547555&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051547555&partnerID=8YFLogxK

U2 - 10.1245/s10434-011-1597-3

DO - 10.1245/s10434-011-1597-3

M3 - Article

C2 - 21347788

AN - SCOPUS:80051547555

VL - 18

SP - 2395

EP - 2403

JO - Annals of Surgical Oncology

JF - Annals of Surgical Oncology

SN - 1068-9265

IS - 8

ER -