Glucose reduction prevents replicative senescence and increases mitochondrial respiration in human mesenchymal stem cells

Ting Lo, Jennifer H. Ho, Muh Hwa Yang, Oscar K. Lee

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The unique self-renewal and multilineage differentiation potential of mesenchymal stem cells (MSCs) make them a promising candidate for cell therapy applications. However, during in vitro expansion of MSCs, replicative senescence may occur and will compromise the quality of the expanded cells. Because calorie restriction has been shown to effectively extend the life span of various organisms, the purpose of this study is to investigate the effect of glucose reduction on MSCs and the coordinated changes in energy utilization. It was found that the frequency of cycling cells was significantly increased, while senescence markers such as β-galactosidase activities and p16 INK4a expression level were markedly reduced in MSCs under low-glucose culture condition. Quantitative real-time PCR analysis demonstrated the preserved trilineage differentiation potentials of MSCs after low-glucose treatment. Interestingly, the ability of osteogenic lineage commitment was improved, while the ability of adipogenic lineage commitment was delayed in MSCs after glucose reduction. In addition, we observed decreased lactate production, increased electron transport chain complexes expression, and increased oxygen consumption in MSCs after glucose reduction treatment. Increased antioxidant defensive responses were evidenced by increased antioxidant enzymes expression and decreased superoxide production after glucose reduction. Taken together, our findings suggest that MSCs utilize energy more efficiently under restricted glucose treatment and exhibit greater self-renewal and antisenescence abilities, while their differentiation potentials remain unaffected.

Original languageEnglish
Pages (from-to)813-825
Number of pages13
JournalCell Transplantation
Volume20
Issue number6
DOIs
Publication statusPublished - 2011

Fingerprint

Cell Aging
Stem cells
Mesenchymal Stromal Cells
Glucose
Respiration
Antioxidants
Galactosidases
Cell- and Tissue-Based Therapy
Electron Transport
Cell culture
Oxygen Consumption
Superoxides
Real-Time Polymerase Chain Reaction
Lactic Acid
Therapeutics
Energy utilization
Enzymes
Oxygen

Keywords

  • Glucose reduction
  • Mesenchymal stem cells (MSCs)
  • Mitochondria
  • Replicative senescence

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

Cite this

Glucose reduction prevents replicative senescence and increases mitochondrial respiration in human mesenchymal stem cells. / Lo, Ting; Ho, Jennifer H.; Yang, Muh Hwa; Lee, Oscar K.

In: Cell Transplantation, Vol. 20, No. 6, 2011, p. 813-825.

Research output: Contribution to journalArticle

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