Glucose biosensor based on glucose oxidase immobilized at gold nanoparticles decorated graphene-carbon nanotubes

Rajkumar Devasenathipathy, Veerappan Mani, Shen Ming Chen, Sheng Tung Huang, Tsung Tao Huang, Chun Mao Lin, Kuo Yuan Hwa, Ting Yo Chen, Bo Jun Chen

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68 Citations (Scopus)

Abstract

Biopolymer pectin stabilized gold nanoparticles were prepared at graphene and multiwalled carbon nanotubes (GR-MWNTs/AuNPs) and employed for the determination of glucose. The formation of GR-MWNTs/AuNPs was confirmed by scanning electron microscopy, X-ray diffraction, UV-vis and FTIR spectroscopy methods. Glucose oxidase (GOx) was successfully immobilized on GR-MWNTs/AuNPs film and direct electron transfer of GOx was investigated. GOx exhibits highly enhanced redox peaks with formal potential of -0.40V (vs. Ag/AgCl). The amount of electroactive GOx and electron transfer rate constant were found to be 10.5×10-10molcm-2 and 3.36s-1, respectively, which were significantly larger than the previous reports. The fabricated amperometric glucose biosensor sensitively detects glucose and showed two linear ranges: (1) 10μM-2mM with LOD of 4.1μM, (2) 2mM-5.2mM with LOD of 0.95mM. The comparison of the biosensor performance with reported sensors reveals the significant improvement in overall sensor performance. Moreover, the biosensor exhibited appreciable stability, repeatability, reproducibility and practicality. The other advantages of the fabricated biosensor are simple and green fabrication approach, roughed and stable electrode surface, fast in sensing and highly reproducible.

Original languageEnglish
Pages (from-to)40-45
Number of pages6
JournalEnzyme and Microbial Technology
Volume78
DOIs
Publication statusPublished - Oct 1 2015

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Keywords

  • Biosensor
  • Carbon nanotubes
  • Electrochemistry
  • Glucose oxidase
  • Gold nanoparticles
  • Graphene

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Devasenathipathy, R., Mani, V., Chen, S. M., Huang, S. T., Huang, T. T., Lin, C. M., Hwa, K. Y., Chen, T. Y., & Chen, B. J. (2015). Glucose biosensor based on glucose oxidase immobilized at gold nanoparticles decorated graphene-carbon nanotubes. Enzyme and Microbial Technology, 78, 40-45. https://doi.org/10.1016/j.enzmictec.2015.06.006