Glow discharge plasma treatment on zirconia surface to enhance osteoblastic-like cell differentiation and antimicrobial effects

Yu Hwa Pan, Jerry Chin Yi Lin, Mei Kuang Chen, Eisner Salamanca, Cheuk Sing Choy, Pei Yo Tsai, Sy Jye Leu, Kai Chiang Yang, Haw Ming Huang, Wan Ling Yao, Wei Jen Chang

Research output: Contribution to journalArticlepeer-review

Abstract

Peri-implantitis is the pathological condition of connective tissue inflammation and the progressive loss of supporting bone around dental implants. One of the primary causes of peri mucositis evolving into peri-implantitis is bacterial infection, including infection from Porphyromonas gingivalis. Enhancing the surface smoothness of implants helps to prevent P. gingivalis adhesion to the implant's surface. Interaction analyses between bacteria and the surface roughness of zirconia (Zr) discs subjected to a glow discharge plasma (GDP) treatment compared with non-plasma-treated autoclaved control Zr discs were done. Examinations of the material prosperities revealed that the GDP-treated Zr group had a smoother surface for a better wettability. The GDP-treated Zr discs improved the proliferation of the osteoblast-like cells MG-63, and the osteoblastic differentiation was assessed through alkaline phosphatase detection and marker gene bone sialoprotein (Bsp) and osteocalcin (OC) induction. Scanning electron microscopy demonstrated a relatively low P. gingivalis adhesion on GDP-treated Zr disks, as well as lower colonization of P. gingivalis compared with the control. Our findings confirmed that the GDP treatment of Zr discs resulted in a significant reduction of P. gingivalis adhesion and growth, demonstrating a positive correlation between surface roughness and bacteria adhesion. Therefore, the GDP treatment of Zr dental implants can provide a method for reducing the risk of peri-implantitis.

Original languageEnglish
Article number3771
JournalMaterials
Volume13
Issue number17
DOIs
Publication statusPublished - Sep 2020

Keywords

  • Glow discharge plasma
  • Osteoblastic-like cell differentiation
  • Peri-implantitis
  • Zirconia

ASJC Scopus subject areas

  • Materials Science(all)

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