Global analysis of differentially expressed genes in early gestational decidua and chorionic villi using a 9600 human cDNA microarray

Huei Wen Chen, Jeremy J W Chen, Chii Ruey Tzeng, Han Ni Li, Shu Ju Chang, Yu Fei Cheng, Ching-Wen Chang, Ruey Sheng Wang, Pan Chyr Yang, Yuan Teh Lee

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The global gene expression profiles of the decidua and chorionic villi of early human pregnancies were analysed by using cDNA microarray technology. Decidual and villous placental tissues were obtained from first trimester abortus and mRNA was extracted for cDNA microarray analysis. The human cDNA microarray [9600 clones, including known regulatory genes and expressed sequence tags (EST)] with colorimetric detection was used to identify differentially expressed genes between early gestational decidua and villi. According to cDNA microarray analysis, we have identified 641 genes with highly expressed mRNA in both decidua and villi, 49 genes with higher expressions in decidua, and 75 genes with higher expression in chorionic villi. These differentially expressed genes were further grouped into categories by their putative functions, including: cell growth-related factors, hormones/cytokines, cell adhesion molecules, signal transduction molecules, apoptosis-related factors, cytoskeleton/extracellular matrix proteins, and EST. Immunohistochemical stainings of cathepsin L, leukaemia inhibitory factor-receptor, and proliferative cell nuclear antigen showed results consistent with the microarray data. Identification of the differentially expressed genes between decidua and villi by microarray provide a global profiling of the gene expression pattern. This work adds to our understanding of placentation by reporting the gene expression profiles during first trimester human pregnancies using cDNA microarray.

Original languageEnglish
Pages (from-to)475-484
Number of pages10
JournalMolecular Human Reproduction
Volume8
Issue number5
Publication statusPublished - 2002

Fingerprint

Chorionic Villi
Decidua
Oligonucleotide Array Sequence Analysis
Genes
Expressed Sequence Tags
First Pregnancy Trimester
Microarray Analysis
Transcriptome
OSM-LIF Receptors
Cathepsin L
Placentation
Nuclear Antigens
Messenger RNA
Extracellular Matrix Proteins
Cell Adhesion Molecules
Gene Expression Profiling
Regulator Genes
Cytoskeleton
Signal Transduction
Intercellular Signaling Peptides and Proteins

Keywords

  • Chorionic villi
  • Decidua
  • First trimester
  • Gene expression
  • Microarray

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Genetics
  • Developmental Biology
  • Embryology
  • Cell Biology

Cite this

Global analysis of differentially expressed genes in early gestational decidua and chorionic villi using a 9600 human cDNA microarray. / Chen, Huei Wen; Chen, Jeremy J W; Tzeng, Chii Ruey; Li, Han Ni; Chang, Shu Ju; Cheng, Yu Fei; Chang, Ching-Wen; Wang, Ruey Sheng; Yang, Pan Chyr; Lee, Yuan Teh.

In: Molecular Human Reproduction, Vol. 8, No. 5, 2002, p. 475-484.

Research output: Contribution to journalArticle

Chen, Huei Wen ; Chen, Jeremy J W ; Tzeng, Chii Ruey ; Li, Han Ni ; Chang, Shu Ju ; Cheng, Yu Fei ; Chang, Ching-Wen ; Wang, Ruey Sheng ; Yang, Pan Chyr ; Lee, Yuan Teh. / Global analysis of differentially expressed genes in early gestational decidua and chorionic villi using a 9600 human cDNA microarray. In: Molecular Human Reproduction. 2002 ; Vol. 8, No. 5. pp. 475-484.
@article{562f8c7091f44a6893606c56bc8b7313,
title = "Global analysis of differentially expressed genes in early gestational decidua and chorionic villi using a 9600 human cDNA microarray",
abstract = "The global gene expression profiles of the decidua and chorionic villi of early human pregnancies were analysed by using cDNA microarray technology. Decidual and villous placental tissues were obtained from first trimester abortus and mRNA was extracted for cDNA microarray analysis. The human cDNA microarray [9600 clones, including known regulatory genes and expressed sequence tags (EST)] with colorimetric detection was used to identify differentially expressed genes between early gestational decidua and villi. According to cDNA microarray analysis, we have identified 641 genes with highly expressed mRNA in both decidua and villi, 49 genes with higher expressions in decidua, and 75 genes with higher expression in chorionic villi. These differentially expressed genes were further grouped into categories by their putative functions, including: cell growth-related factors, hormones/cytokines, cell adhesion molecules, signal transduction molecules, apoptosis-related factors, cytoskeleton/extracellular matrix proteins, and EST. Immunohistochemical stainings of cathepsin L, leukaemia inhibitory factor-receptor, and proliferative cell nuclear antigen showed results consistent with the microarray data. Identification of the differentially expressed genes between decidua and villi by microarray provide a global profiling of the gene expression pattern. This work adds to our understanding of placentation by reporting the gene expression profiles during first trimester human pregnancies using cDNA microarray.",
keywords = "Chorionic villi, Decidua, First trimester, Gene expression, Microarray",
author = "Chen, {Huei Wen} and Chen, {Jeremy J W} and Tzeng, {Chii Ruey} and Li, {Han Ni} and Chang, {Shu Ju} and Cheng, {Yu Fei} and Ching-Wen Chang and Wang, {Ruey Sheng} and Yang, {Pan Chyr} and Lee, {Yuan Teh}",
year = "2002",
language = "English",
volume = "8",
pages = "475--484",
journal = "Molecular Human Reproduction",
issn = "1360-9947",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - Global analysis of differentially expressed genes in early gestational decidua and chorionic villi using a 9600 human cDNA microarray

AU - Chen, Huei Wen

AU - Chen, Jeremy J W

AU - Tzeng, Chii Ruey

AU - Li, Han Ni

AU - Chang, Shu Ju

AU - Cheng, Yu Fei

AU - Chang, Ching-Wen

AU - Wang, Ruey Sheng

AU - Yang, Pan Chyr

AU - Lee, Yuan Teh

PY - 2002

Y1 - 2002

N2 - The global gene expression profiles of the decidua and chorionic villi of early human pregnancies were analysed by using cDNA microarray technology. Decidual and villous placental tissues were obtained from first trimester abortus and mRNA was extracted for cDNA microarray analysis. The human cDNA microarray [9600 clones, including known regulatory genes and expressed sequence tags (EST)] with colorimetric detection was used to identify differentially expressed genes between early gestational decidua and villi. According to cDNA microarray analysis, we have identified 641 genes with highly expressed mRNA in both decidua and villi, 49 genes with higher expressions in decidua, and 75 genes with higher expression in chorionic villi. These differentially expressed genes were further grouped into categories by their putative functions, including: cell growth-related factors, hormones/cytokines, cell adhesion molecules, signal transduction molecules, apoptosis-related factors, cytoskeleton/extracellular matrix proteins, and EST. Immunohistochemical stainings of cathepsin L, leukaemia inhibitory factor-receptor, and proliferative cell nuclear antigen showed results consistent with the microarray data. Identification of the differentially expressed genes between decidua and villi by microarray provide a global profiling of the gene expression pattern. This work adds to our understanding of placentation by reporting the gene expression profiles during first trimester human pregnancies using cDNA microarray.

AB - The global gene expression profiles of the decidua and chorionic villi of early human pregnancies were analysed by using cDNA microarray technology. Decidual and villous placental tissues were obtained from first trimester abortus and mRNA was extracted for cDNA microarray analysis. The human cDNA microarray [9600 clones, including known regulatory genes and expressed sequence tags (EST)] with colorimetric detection was used to identify differentially expressed genes between early gestational decidua and villi. According to cDNA microarray analysis, we have identified 641 genes with highly expressed mRNA in both decidua and villi, 49 genes with higher expressions in decidua, and 75 genes with higher expression in chorionic villi. These differentially expressed genes were further grouped into categories by their putative functions, including: cell growth-related factors, hormones/cytokines, cell adhesion molecules, signal transduction molecules, apoptosis-related factors, cytoskeleton/extracellular matrix proteins, and EST. Immunohistochemical stainings of cathepsin L, leukaemia inhibitory factor-receptor, and proliferative cell nuclear antigen showed results consistent with the microarray data. Identification of the differentially expressed genes between decidua and villi by microarray provide a global profiling of the gene expression pattern. This work adds to our understanding of placentation by reporting the gene expression profiles during first trimester human pregnancies using cDNA microarray.

KW - Chorionic villi

KW - Decidua

KW - First trimester

KW - Gene expression

KW - Microarray

UR - http://www.scopus.com/inward/record.url?scp=18444398948&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18444398948&partnerID=8YFLogxK

M3 - Article

C2 - 11994545

AN - SCOPUS:18444398948

VL - 8

SP - 475

EP - 484

JO - Molecular Human Reproduction

JF - Molecular Human Reproduction

SN - 1360-9947

IS - 5

ER -