Glial cell adhesive molecule unzipped mediates axon guidance in Drosophila

Zhao Ying Ding, Ying Hsuan Wang, Zhao Kai Luo, Huei Fang Lee, Jaulang Hwang, Cheng Ting Chien, Min Lang Huang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Axon guidance needs help from the glial cell system during embryogenesis. In the Drosophila embryonic central nervous system (CNS), longitudinal glia (LG) have been implicated in axon guidance but the mechanism remains unclear. We identified the protein encoded by the Drosophila gene unzipped (uzip) as a novel cell adhesion molecule (CAM). Uzip expressed in Drosophila S2 cells triggered cell aggregation through homophilic binding. In the embryonic CNS, Uzip was mainly produced by the LG but was also located at axons, which is consistent with the secretion of Uzip expressed in cultured cells. Although uzip mutants displayed no axonal defect, loss of uzip enhanced the axonal defects in the mutant of N-cadherin (CadN) and the Wnt gene family member wnt5. Overexpression of uzip could rescue the phenotype in the CadNuzipD43 mutant. Thus, Uzip is a novel CAM from the LG regulating axon guidance.

Original languageEnglish
Pages (from-to)122-134
Number of pages13
JournalDevelopmental Dynamics
Volume240
Issue number1
DOIs
Publication statusPublished - Jan 2011
Externally publishedYes

Fingerprint

Neuroglia
Adhesives
Drosophila
Cell Adhesion Molecules
Central Nervous System
Drosophila Proteins
Cell Aggregation
Cadherins
Genes
Embryonic Development
Axons
Cultured Cells
Phenotype
Axon Guidance

Keywords

  • Adhesion
  • Axon guidance
  • CAM
  • Drosophila
  • Glia
  • Unzipped

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Ding, Z. Y., Wang, Y. H., Luo, Z. K., Lee, H. F., Hwang, J., Chien, C. T., & Huang, M. L. (2011). Glial cell adhesive molecule unzipped mediates axon guidance in Drosophila. Developmental Dynamics, 240(1), 122-134. https://doi.org/10.1002/dvdy.22508

Glial cell adhesive molecule unzipped mediates axon guidance in Drosophila. / Ding, Zhao Ying; Wang, Ying Hsuan; Luo, Zhao Kai; Lee, Huei Fang; Hwang, Jaulang; Chien, Cheng Ting; Huang, Min Lang.

In: Developmental Dynamics, Vol. 240, No. 1, 01.2011, p. 122-134.

Research output: Contribution to journalArticle

Ding, ZY, Wang, YH, Luo, ZK, Lee, HF, Hwang, J, Chien, CT & Huang, ML 2011, 'Glial cell adhesive molecule unzipped mediates axon guidance in Drosophila', Developmental Dynamics, vol. 240, no. 1, pp. 122-134. https://doi.org/10.1002/dvdy.22508
Ding ZY, Wang YH, Luo ZK, Lee HF, Hwang J, Chien CT et al. Glial cell adhesive molecule unzipped mediates axon guidance in Drosophila. Developmental Dynamics. 2011 Jan;240(1):122-134. https://doi.org/10.1002/dvdy.22508
Ding, Zhao Ying ; Wang, Ying Hsuan ; Luo, Zhao Kai ; Lee, Huei Fang ; Hwang, Jaulang ; Chien, Cheng Ting ; Huang, Min Lang. / Glial cell adhesive molecule unzipped mediates axon guidance in Drosophila. In: Developmental Dynamics. 2011 ; Vol. 240, No. 1. pp. 122-134.
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