Ginseng Flowers Stimulate Progesterone Production from Bovine Luteal Cells

Leang Shin Wu, Shiow Yunn Sheu, Cheng Chun Huang, Chih Hsien Chiu, Jan Chi Huang, Jenn Rong Yang, Wai Xiong Lian, Chih Hao Lai, Yuh Pan Chen, Jen Hsou Lin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Our previous report first showed evidence that polysaccharides isolated from ginseng leaves obtained from Jilin, China possess luteotropic activities. In this study, we made further investigations on the root and flowers of Korean ginseng by means of the same bioassay system described briefly as follows. Frozen-thawed bovine luteal cells (1 × 105 cells/ml/well) in M199 were incubated in 24-well culture plates at 37°C in a 5 % CO2 incubator. Ten μl of tested drugs with 1,10 and 100 μg/ml were added into each well. After 4- and 24-hr incubation, the media were harvested and assayed for progesterone by an enzyme immunoassay. The production of progesterone from cells is the indicator for evaluating the action of tested drugs. Results showed that hot water extracts of ginseng flowers (GF-1 ) with 10 to 100 μg/ml significantly increased progesterone production, whereas those from ginseng root (GR-1) could not. Crude polysaccharides (GF-2) isolated from GF-1 is the active component and the small molecules (mw <10,000 dalton) are excluded, indicating that the ginseng root has no luteotropic activities, but the polysaccharides of ginseng flowers have.

Original languageEnglish
Pages (from-to)371-377
Number of pages7
JournalAmerican Journal of Chinese Medicine
Volume28
Issue number3-4
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Luteal Cells
Panax
Progesterone
Polysaccharides
Incubators
Immunoenzyme Techniques
Biological Assay
Pharmaceutical Preparations
China
Water

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Wu, L. S., Sheu, S. Y., Huang, C. C., Chiu, C. H., Huang, J. C., Yang, J. R., ... Lin, J. H. (2000). Ginseng Flowers Stimulate Progesterone Production from Bovine Luteal Cells. American Journal of Chinese Medicine, 28(3-4), 371-377.

Ginseng Flowers Stimulate Progesterone Production from Bovine Luteal Cells. / Wu, Leang Shin; Sheu, Shiow Yunn; Huang, Cheng Chun; Chiu, Chih Hsien; Huang, Jan Chi; Yang, Jenn Rong; Lian, Wai Xiong; Lai, Chih Hao; Chen, Yuh Pan; Lin, Jen Hsou.

In: American Journal of Chinese Medicine, Vol. 28, No. 3-4, 2000, p. 371-377.

Research output: Contribution to journalArticle

Wu, LS, Sheu, SY, Huang, CC, Chiu, CH, Huang, JC, Yang, JR, Lian, WX, Lai, CH, Chen, YP & Lin, JH 2000, 'Ginseng Flowers Stimulate Progesterone Production from Bovine Luteal Cells', American Journal of Chinese Medicine, vol. 28, no. 3-4, pp. 371-377.
Wu, Leang Shin ; Sheu, Shiow Yunn ; Huang, Cheng Chun ; Chiu, Chih Hsien ; Huang, Jan Chi ; Yang, Jenn Rong ; Lian, Wai Xiong ; Lai, Chih Hao ; Chen, Yuh Pan ; Lin, Jen Hsou. / Ginseng Flowers Stimulate Progesterone Production from Bovine Luteal Cells. In: American Journal of Chinese Medicine. 2000 ; Vol. 28, No. 3-4. pp. 371-377.
@article{969957f062004aeda4d977a906b4567b,
title = "Ginseng Flowers Stimulate Progesterone Production from Bovine Luteal Cells",
abstract = "Our previous report first showed evidence that polysaccharides isolated from ginseng leaves obtained from Jilin, China possess luteotropic activities. In this study, we made further investigations on the root and flowers of Korean ginseng by means of the same bioassay system described briefly as follows. Frozen-thawed bovine luteal cells (1 × 105 cells/ml/well) in M199 were incubated in 24-well culture plates at 37°C in a 5 {\%} CO2 incubator. Ten μl of tested drugs with 1,10 and 100 μg/ml were added into each well. After 4- and 24-hr incubation, the media were harvested and assayed for progesterone by an enzyme immunoassay. The production of progesterone from cells is the indicator for evaluating the action of tested drugs. Results showed that hot water extracts of ginseng flowers (GF-1 ) with 10 to 100 μg/ml significantly increased progesterone production, whereas those from ginseng root (GR-1) could not. Crude polysaccharides (GF-2) isolated from GF-1 is the active component and the small molecules (mw <10,000 dalton) are excluded, indicating that the ginseng root has no luteotropic activities, but the polysaccharides of ginseng flowers have.",
author = "Wu, {Leang Shin} and Sheu, {Shiow Yunn} and Huang, {Cheng Chun} and Chiu, {Chih Hsien} and Huang, {Jan Chi} and Yang, {Jenn Rong} and Lian, {Wai Xiong} and Lai, {Chih Hao} and Chen, {Yuh Pan} and Lin, {Jen Hsou}",
year = "2000",
language = "English",
volume = "28",
pages = "371--377",
journal = "American Journal of Chinese Medicine",
issn = "0192-415X",
publisher = "World Scientific Publishing Co. Pte Ltd",
number = "3-4",

}

TY - JOUR

T1 - Ginseng Flowers Stimulate Progesterone Production from Bovine Luteal Cells

AU - Wu, Leang Shin

AU - Sheu, Shiow Yunn

AU - Huang, Cheng Chun

AU - Chiu, Chih Hsien

AU - Huang, Jan Chi

AU - Yang, Jenn Rong

AU - Lian, Wai Xiong

AU - Lai, Chih Hao

AU - Chen, Yuh Pan

AU - Lin, Jen Hsou

PY - 2000

Y1 - 2000

N2 - Our previous report first showed evidence that polysaccharides isolated from ginseng leaves obtained from Jilin, China possess luteotropic activities. In this study, we made further investigations on the root and flowers of Korean ginseng by means of the same bioassay system described briefly as follows. Frozen-thawed bovine luteal cells (1 × 105 cells/ml/well) in M199 were incubated in 24-well culture plates at 37°C in a 5 % CO2 incubator. Ten μl of tested drugs with 1,10 and 100 μg/ml were added into each well. After 4- and 24-hr incubation, the media were harvested and assayed for progesterone by an enzyme immunoassay. The production of progesterone from cells is the indicator for evaluating the action of tested drugs. Results showed that hot water extracts of ginseng flowers (GF-1 ) with 10 to 100 μg/ml significantly increased progesterone production, whereas those from ginseng root (GR-1) could not. Crude polysaccharides (GF-2) isolated from GF-1 is the active component and the small molecules (mw <10,000 dalton) are excluded, indicating that the ginseng root has no luteotropic activities, but the polysaccharides of ginseng flowers have.

AB - Our previous report first showed evidence that polysaccharides isolated from ginseng leaves obtained from Jilin, China possess luteotropic activities. In this study, we made further investigations on the root and flowers of Korean ginseng by means of the same bioassay system described briefly as follows. Frozen-thawed bovine luteal cells (1 × 105 cells/ml/well) in M199 were incubated in 24-well culture plates at 37°C in a 5 % CO2 incubator. Ten μl of tested drugs with 1,10 and 100 μg/ml were added into each well. After 4- and 24-hr incubation, the media were harvested and assayed for progesterone by an enzyme immunoassay. The production of progesterone from cells is the indicator for evaluating the action of tested drugs. Results showed that hot water extracts of ginseng flowers (GF-1 ) with 10 to 100 μg/ml significantly increased progesterone production, whereas those from ginseng root (GR-1) could not. Crude polysaccharides (GF-2) isolated from GF-1 is the active component and the small molecules (mw <10,000 dalton) are excluded, indicating that the ginseng root has no luteotropic activities, but the polysaccharides of ginseng flowers have.

UR - http://www.scopus.com/inward/record.url?scp=0034567331&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034567331&partnerID=8YFLogxK

M3 - Article

C2 - 11154050

AN - SCOPUS:0034567331

VL - 28

SP - 371

EP - 377

JO - American Journal of Chinese Medicine

JF - American Journal of Chinese Medicine

SN - 0192-415X

IS - 3-4

ER -