Ginkgolide C suppresses adipogenesis in 3T3-L1 adipocytes via the AMPK signaling pathway

Chian Jiun Liou, Xuan Yu Lai, Ya Ling Chen, Chia Ling Wang, Ciao Han Wei, Wen Chung Huang

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Ginkgolide C, isolated from Ginkgo biloba leaves, is a flavone reported to have multiple biological functions, from decreased platelet aggregation to ameliorating Alzheimer disease. The study aim was to evaluate the antiadipogenic effect of ginkgolide C in 3T3-L1 adipocytes. Ginkgolide C was used to treat differentiated 3T3-L1 cells. Cell supernatant was collected to assay glycerol release, and cells were lysed to measure protein and gene expression related to adipogenesis and lipolysis by western blot and real-time PCR, respectively. Ginkgolide C significantly suppressed lipid accumulation in differentiated adipocytes. It also decreased adipogenesis-related transcription factor expression, including peroxisome proliferator-activated receptor and CCAAT/enhancer-binding protein. Furthermore, ginkgolide C enhanced adipose triglyceride lipase and hormone-sensitive lipase production for lipolysis and increased phosphorylation of AMP-activated protein kinase (AMPK), resulting in decreased activity of acetyl-CoA carboxylase for fatty acid synthesis. In coculture with an AMPK inhibitor (compound C), ginkgolide C also improved activation of sirtuin 1 and phosphorylation of AMPK in differentiated 3T3-L1 cells. The results suggest that ginkgolide C is an effective flavone for increasing lipolysis and inhibiting adipogenesis in adipocytes through the activated AMPK pathway.

Original languageEnglish
Article number298635
JournalEvidence-based Complementary and Alternative Medicine
Volume2015
DOIs
Publication statusPublished - Jan 1 2015
Externally publishedYes

ASJC Scopus subject areas

  • Complementary and alternative medicine

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