Ginkgo biloba extract inhibits tumor necrosis factor-α-induced reactive oxygen species generation, transcription factor activation, and cell adhesion molecule expression in human aortic endothelial cells

Jaw Wen Chen, Yung Hsiang Chen, Feng Yan Lin, Yuh Lien Chen, Shing Jong Lin

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Objective - This study was conducted to examination whether Ginkgo biloba extract (GBE), a Chinese herb with antioxidant activity, could reduce cytokine-induced monocyte/human aortic endothelial cell (HAEC) interaction, a pivotal early event in atherogenesis. Methods and Results - Pretreatment of HAECs with GBE (50 and 100 μg/mL for 18 hours) significantly suppressed cellular binding between the human monocytic cell line U937 and tumor necrosis factor-α (TNF-α)-stimulated HAECs by using in vitro binding assay (68.7% and 60.1% inhibitions, respectively). Cell enzyme-linked immunosorbent assay and immunoblot analysis showed that GBE (50 μg/mL for 18 hours) significantly attenuated TNF-α-induced cell surface and total protein expression of vascular cellular adhesion molecule-1 and intracellular adhesion molecule-1 (63.5% and 69.2%, respectively; P

Original languageEnglish
Pages (from-to)1559-1566
Number of pages8
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume23
Issue number9
DOIs
Publication statusPublished - Sep 1 2003
Externally publishedYes

Keywords

  • Activator protein 1
  • Cell adhesion molecule
  • Ginkgo biloba
  • Human aortic endothelial cells
  • Nuclear factor-κB

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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