Giant polypeptides of skeletal muscle titin: Sedimentation equilibrium in guanidine hydrochloride

Gary P. Kurzban, Kuan Wang

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Titin has been purified from rabbit skeletal myofibrils in guanidine hydrochloride by gel filtration on Sephacryl S-1000. The sedimentation coefficients of denatured titin in 6 M guanidine hydrochloride are highly concentration dependent and follow the equation: 1 S = 0.241 + 0.302 A280. Equilibrium sedimentation studies indicate that the average molecular weight of titin polypeptides is 2.4 to 2.6 × 106.

Original languageEnglish
Pages (from-to)1155-1161
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume150
Issue number3
DOIs
Publication statusPublished - Feb 15 1988
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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