Genotype analysis using human hair shaft

Hsueh Wei Chang, Ching Yu Yen, Shyun Yui Liu, Gad Singer, Ie Ming Shih

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Analysis of genotypes for genetic predisposition of diseases has profound and widespread clinical application and has become a fundamental tool in the study of molecular epidemiology. In this study, we report a convenient and completely noninvasive approach to perform genotyping using terminal cephalic hair shaft. The average and median DNA amounts purified from the hair shaft are 112 ± 180 (1 SD) pg/mg and 66 pg/mg of hair shaft, respectively. Using hybridization with molecular beacons that can reliably distinguish single nucleotide polymorphisms at different alleles, we are able to analyze the genotypes of APO-E and G-protein β3 (GNB3) subunit C825T loci in DNA samples obtained from hair shaft in a high throughput format. The amount of DNA obtained from 20 mg of hair shaft is sufficient for multiple independent genotyping assays. These findings demonstrate that terminal hair shaft provides a convenient and reliable genomic DNA source for an analysis of genotypes.

Original languageEnglish
Pages (from-to)925-929
Number of pages5
JournalCancer Epidemiology Biomarkers and Prevention
Volume11
Issue number9
Publication statusPublished - Sep 2002
Externally publishedYes

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Hair
Genotype
DNA
Inborn Genetic Diseases
Molecular Epidemiology
Genetic Predisposition to Disease
GTP-Binding Proteins
Single Nucleotide Polymorphism
Alleles
Head

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Chang, H. W., Yen, C. Y., Liu, S. Y., Singer, G., & Shih, I. M. (2002). Genotype analysis using human hair shaft. Cancer Epidemiology Biomarkers and Prevention, 11(9), 925-929.

Genotype analysis using human hair shaft. / Chang, Hsueh Wei; Yen, Ching Yu; Liu, Shyun Yui; Singer, Gad; Shih, Ie Ming.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 11, No. 9, 09.2002, p. 925-929.

Research output: Contribution to journalArticle

Chang, HW, Yen, CY, Liu, SY, Singer, G & Shih, IM 2002, 'Genotype analysis using human hair shaft', Cancer Epidemiology Biomarkers and Prevention, vol. 11, no. 9, pp. 925-929.
Chang HW, Yen CY, Liu SY, Singer G, Shih IM. Genotype analysis using human hair shaft. Cancer Epidemiology Biomarkers and Prevention. 2002 Sep;11(9):925-929.
Chang, Hsueh Wei ; Yen, Ching Yu ; Liu, Shyun Yui ; Singer, Gad ; Shih, Ie Ming. / Genotype analysis using human hair shaft. In: Cancer Epidemiology Biomarkers and Prevention. 2002 ; Vol. 11, No. 9. pp. 925-929.
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