Genotype analysis using human hair shaft

Hsueh Wei Chang, Ching Yu Yen, Shyun Yui Liu, Gad Singer, Ie Ming Shih

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Analysis of genotypes for genetic predisposition of diseases has profound and widespread clinical application and has become a fundamental tool in the study of molecular epidemiology. In this study, we report a convenient and completely noninvasive approach to perform genotyping using terminal cephalic hair shaft. The average and median DNA amounts purified from the hair shaft are 112 ± 180 (1 SD) pg/mg and 66 pg/mg of hair shaft, respectively. Using hybridization with molecular beacons that can reliably distinguish single nucleotide polymorphisms at different alleles, we are able to analyze the genotypes of APO-E and G-protein β3 (GNB3) subunit C825T loci in DNA samples obtained from hair shaft in a high throughput format. The amount of DNA obtained from 20 mg of hair shaft is sufficient for multiple independent genotyping assays. These findings demonstrate that terminal hair shaft provides a convenient and reliable genomic DNA source for an analysis of genotypes.

Original languageEnglish
Pages (from-to)925-929
Number of pages5
JournalCancer Epidemiology Biomarkers and Prevention
Volume11
Issue number9
Publication statusPublished - Sep 2002
Externally publishedYes

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

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  • Cite this

    Chang, H. W., Yen, C. Y., Liu, S. Y., Singer, G., & Shih, I. M. (2002). Genotype analysis using human hair shaft. Cancer Epidemiology Biomarkers and Prevention, 11(9), 925-929.