Genotoxicity of motorcycle exhaust particles in vivo and in vitro

Yu Wen Cheng, Wen Wha Lee, Ching Hao Li, Chen Chen Lee, Jaw Jou Kang

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We studied the genotoxic potency of motorcycle exhaust particles (MEP) by using a bacterial reversion assay and chromosome aberration and micronucleus tests. In the bacterial reversion assay (Ames test), MEP concentration-dependently increased TA98, TA100, and TA102 revertants in the presence of metabolic-activating enzymes. In the chromosome aberration test, MEP concentration-dependently increased abnormal structural chromosomes in CHO-K1 cells both with and without S9. Pretreatment with antioxidants (α-tocopherol, ascorbate, catalase, and NAC) showed varying degrees of inhibitory effect on the MEP-induced mutagenic effect and chromosome structural abnormalities. In the in vivo micronucleus test, MEP dose-dependently induced micronucleus formation in peripheral red blood cells after 24 and 48 h of treatment. The increase of micronucleated reticulocytes induced by MEP was inhibited by pretreatment with α-tocopherol and ascorbate. The fluorescence intensity of DCFH-DA-loaded CHO-K1 cells was increased upon the addition of MEP. Our data suggest that MEP can induce genotoxicity through a reactive oxygen species-(ROS-) dependent pathway, which can be augmented by metabolic activation. Alpha-tocopherol, ascorbate, catalase, and NAC can inhibit MEP-induced genotoxicity, indicating that ROS might be involved in this effect.

Original languageEnglish
Pages (from-to)103-111
Number of pages9
JournalToxicological Sciences
Volume81
Issue number1
DOIs
Publication statusPublished - Sep 2004
Externally publishedYes

Keywords

  • Ames test
  • Chromosome aberration
  • Genotoxicity
  • Micronucleus
  • Motorcycle exhaust particles
  • Reactive oxygen species

ASJC Scopus subject areas

  • Toxicology

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