Genomic structure, expression, and chromosomal localization of the human glycine N-methyltransferase gene

Yi Ming A. Chen, Li Ying Chen, Fen Hwa Wong, Cheng Ming Lee, Tai Jay Chang, Teresa L. Yang-Feng

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The glycine N-methyltransferase (GNMT) gene encodes a protein that not only acts as an enzyme to regulate the ratio of S-adenosylmethionine to S- adenosylhomocysteine, but also participates in the detoxification pathway in liver cells. Previously, we reported that the expression level of GNMT was diminished in human hepatocellular carcinoma. In this study, the human GNMT gene was cloned and characterized. It contains six exons and spans about 10 kb. Instead of a TATA box, it has a transcriptional initiator located 801 bp upstream from the translation start codon. The gene was localized to chromosome 6p12 using fluorescence in situ hybridization. Northern blot analysis of 16 tissues from different human organs showed that GNMT was expressed only in liver, pancreas, and prostate. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)43-47
Number of pages5
JournalGenomics
Volume66
Issue number1
DOIs
Publication statusPublished - May 15 2000
Externally publishedYes

Fingerprint

Glycine N-Methyltransferase
S-Adenosylhomocysteine
Genes
S-Adenosylmethionine
TATA Box
Initiator Codon
Liver
Fluorescence In Situ Hybridization
Northern Blotting
Prostate
Pancreas
Hepatocellular Carcinoma
Exons
Chromosomes
Enzymes
human GNMT protein
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Genomic structure, expression, and chromosomal localization of the human glycine N-methyltransferase gene. / Chen, Yi Ming A.; Chen, Li Ying; Wong, Fen Hwa; Lee, Cheng Ming; Chang, Tai Jay; Yang-Feng, Teresa L.

In: Genomics, Vol. 66, No. 1, 15.05.2000, p. 43-47.

Research output: Contribution to journalArticle

Chen, Yi Ming A. ; Chen, Li Ying ; Wong, Fen Hwa ; Lee, Cheng Ming ; Chang, Tai Jay ; Yang-Feng, Teresa L. / Genomic structure, expression, and chromosomal localization of the human glycine N-methyltransferase gene. In: Genomics. 2000 ; Vol. 66, No. 1. pp. 43-47.
@article{43de127375a342539f80402f83f1235f,
title = "Genomic structure, expression, and chromosomal localization of the human glycine N-methyltransferase gene",
abstract = "The glycine N-methyltransferase (GNMT) gene encodes a protein that not only acts as an enzyme to regulate the ratio of S-adenosylmethionine to S- adenosylhomocysteine, but also participates in the detoxification pathway in liver cells. Previously, we reported that the expression level of GNMT was diminished in human hepatocellular carcinoma. In this study, the human GNMT gene was cloned and characterized. It contains six exons and spans about 10 kb. Instead of a TATA box, it has a transcriptional initiator located 801 bp upstream from the translation start codon. The gene was localized to chromosome 6p12 using fluorescence in situ hybridization. Northern blot analysis of 16 tissues from different human organs showed that GNMT was expressed only in liver, pancreas, and prostate. (C) 2000 Academic Press.",
author = "Chen, {Yi Ming A.} and Chen, {Li Ying} and Wong, {Fen Hwa} and Lee, {Cheng Ming} and Chang, {Tai Jay} and Yang-Feng, {Teresa L.}",
year = "2000",
month = "5",
day = "15",
doi = "10.1006/geno.2000.6188",
language = "English",
volume = "66",
pages = "43--47",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Genomic structure, expression, and chromosomal localization of the human glycine N-methyltransferase gene

AU - Chen, Yi Ming A.

AU - Chen, Li Ying

AU - Wong, Fen Hwa

AU - Lee, Cheng Ming

AU - Chang, Tai Jay

AU - Yang-Feng, Teresa L.

PY - 2000/5/15

Y1 - 2000/5/15

N2 - The glycine N-methyltransferase (GNMT) gene encodes a protein that not only acts as an enzyme to regulate the ratio of S-adenosylmethionine to S- adenosylhomocysteine, but also participates in the detoxification pathway in liver cells. Previously, we reported that the expression level of GNMT was diminished in human hepatocellular carcinoma. In this study, the human GNMT gene was cloned and characterized. It contains six exons and spans about 10 kb. Instead of a TATA box, it has a transcriptional initiator located 801 bp upstream from the translation start codon. The gene was localized to chromosome 6p12 using fluorescence in situ hybridization. Northern blot analysis of 16 tissues from different human organs showed that GNMT was expressed only in liver, pancreas, and prostate. (C) 2000 Academic Press.

AB - The glycine N-methyltransferase (GNMT) gene encodes a protein that not only acts as an enzyme to regulate the ratio of S-adenosylmethionine to S- adenosylhomocysteine, but also participates in the detoxification pathway in liver cells. Previously, we reported that the expression level of GNMT was diminished in human hepatocellular carcinoma. In this study, the human GNMT gene was cloned and characterized. It contains six exons and spans about 10 kb. Instead of a TATA box, it has a transcriptional initiator located 801 bp upstream from the translation start codon. The gene was localized to chromosome 6p12 using fluorescence in situ hybridization. Northern blot analysis of 16 tissues from different human organs showed that GNMT was expressed only in liver, pancreas, and prostate. (C) 2000 Academic Press.

UR - http://www.scopus.com/inward/record.url?scp=0034657925&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034657925&partnerID=8YFLogxK

U2 - 10.1006/geno.2000.6188

DO - 10.1006/geno.2000.6188

M3 - Article

C2 - 10843803

AN - SCOPUS:0034657925

VL - 66

SP - 43

EP - 47

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 1

ER -