Genomic structure, expression and characterization of a STAT5 homologue from pufferfish (Tetraodon fluviatilis)

Shu Chiun Sung, Ting Jia Fan, Chih Ming Chou, Jiann Horng Leu, Ya Li Hsu, Shui Tsung Chen, Yueh Chun Hsieh, Chang Jen Huang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The STAT5 (signal transducer and activator of transcription 5) gene was isolated and characterized from a round-spotted pufferfish genomic library. This gene is composed of 19 exons spanning 11 kb. The full-length cDNA of Tetraodon fluviatilis STAT5 (TfSTAT5) contains 2461 bp and encodes a protein of 785 amino acid residues. From the amino acid sequence comparison, TfSTAT5 is most similar to mouse STAT5a and STAT5b with an overall identity of 76% and 78%, respectively, and has <35% identity with other mammalian STATs. The exon/intron junctions of the TfSTAT5 gene were almost identical to those of mouse STAT5a and STAT5b genes, indicating that these genes are highly conserved at the levels of amino acid sequence and genomic structure. To understand better the biochemical properties of TfSTAT5, a chimeric STAT5 was generated by fusion of the kinase-catalytic domain of carp Janus kinase 1 (JAK1) to the C-terminal end of TfSTAT5. The fusion protein was expressed and tyrosine-phosphorylated by its kinase domain. The fusion protein exhibits specific DNA-binding and transactivation potential toward an artificial fish promoter as well as authentic mammalian promoters such as the β-casein promoter and cytokine inducible SH2 containing protein (CIS) promoter when expressed in both fish and mammalian cells. However, TfSTAT5 could not induce the transcription of β-casein promoter via rat prolactin and Nb2 prolactin receptor. To our knowledge, this is the first report describing detailed biochemical characterization of a STAT protein from fish.

Original languageEnglish
Pages (from-to)239-252
Number of pages14
JournalEuropean Journal of Biochemistry
Volume270
Issue number2
DOIs
Publication statusPublished - 2003

Fingerprint

STAT5 Transcription Factor
Tetraodontiformes
Genes
Fusion reactions
Caseins
Amino Acids
Fish
Amino Acid Sequence
Exons
Janus Kinase 1
Fishes
Phosphotransferases
Fish Proteins
Prolactin Receptors
Proteins
Genomic Library
Carps
Transcription
Prolactin
Introns

Keywords

  • DNA binding
  • Pufferfish
  • Signal transduction
  • STAT5
  • Transactivation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Genomic structure, expression and characterization of a STAT5 homologue from pufferfish (Tetraodon fluviatilis). / Sung, Shu Chiun; Fan, Ting Jia; Chou, Chih Ming; Leu, Jiann Horng; Hsu, Ya Li; Chen, Shui Tsung; Hsieh, Yueh Chun; Huang, Chang Jen.

In: European Journal of Biochemistry, Vol. 270, No. 2, 2003, p. 239-252.

Research output: Contribution to journalArticle

Sung, Shu Chiun ; Fan, Ting Jia ; Chou, Chih Ming ; Leu, Jiann Horng ; Hsu, Ya Li ; Chen, Shui Tsung ; Hsieh, Yueh Chun ; Huang, Chang Jen. / Genomic structure, expression and characterization of a STAT5 homologue from pufferfish (Tetraodon fluviatilis). In: European Journal of Biochemistry. 2003 ; Vol. 270, No. 2. pp. 239-252.
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