Genomic organization and the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) CDC37 gene

Shu Chuan Tsai, Jiann Horng Leu, Chih Ming Chou, Maw Sheng Yeh, Fore Lien Huang, Chang Jen Huang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The CDC37 gene was isolated from a round-spotted pufferfish genomic library and characterized. This gene is composed of nine exons spanning 3.5 kb. Exon 1 contains the 5'-untranslated region and exon 2 contains the putative translation initiation site. By 5'-RACE (rapid amplication of cDNA ends) and sequence analysis, we deduced the promoter region for the CDC37 gene and found that it does not contain typical TATA or CCAAT box. The 1.8 kb DNA fragment upstream of the putative transcription initiation site contains numerous potential binding sites for transcription factors including CREB, E2A, Ets-1, GATA, NF-IL6 and PEA3. When this DNA fragment was placed upstream of the chloramphenicol acetyltransferase (CAT) reporter gene and transfected into a carp CF cell line, it could drive the synthesis of CAT enzyme four times more efficiently than the promoterless pCAT-Basic did. In addition, the CDC37 gene is linked to the TYK2 gene in a tail-to-head manner with a small intergenic region of 292 bp. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)144-148
Number of pages5
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1494
Issue number1-2
DOIs
Publication statusPublished - Nov 15 2000
Externally publishedYes

Fingerprint

Tetraodontiformes
Genetic Promoter Regions
Genes
Exons
Chloramphenicol O-Acetyltransferase
CCAAT-Enhancer-Binding Protein-beta
Intergenic DNA
Genomic Library
Carps
5' Untranslated Regions
Transcription Initiation Site
DNA
Reporter Genes
Sequence Analysis
Tail
Transcription Factors
Complementary DNA
Binding Sites
Cell Line
Cells

Keywords

  • CDC37
  • Genomic structure
  • Promoter
  • Round-spotted pufferfish (Tetraodon fluviatilis)

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Genomic organization and the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) CDC37 gene. / Tsai, Shu Chuan; Leu, Jiann Horng; Chou, Chih Ming; Yeh, Maw Sheng; Huang, Fore Lien; Huang, Chang Jen.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1494, No. 1-2, 15.11.2000, p. 144-148.

Research output: Contribution to journalArticle

Tsai, Shu Chuan ; Leu, Jiann Horng ; Chou, Chih Ming ; Yeh, Maw Sheng ; Huang, Fore Lien ; Huang, Chang Jen. / Genomic organization and the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) CDC37 gene. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 2000 ; Vol. 1494, No. 1-2. pp. 144-148.
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