Genome-wide expression profiles of subchondral bone in osteoarthritis

Ching Heng Chou, Chia Chun Wu, I. Wen Song, Hui Ping Chuang, Liang Suei Lu, Jen Huei Chang, San Yuan Kuo, Chian Her Lee, Jer Yuarn Wu, Yuan Tsong Chen, Virginia B. Kraus, Ming Ta Michael Lee

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52 Citations (Scopus)

Abstract

Introduction: The aim of this study was to evaluate, for the first time, the differences in gene expression profiles of normal and osteoarthritic (OA) subchondral bone in human subjects.Methods: Following histological assessment of the integrity of overlying cartilage and the severity of bone abnormality by micro-computed tomography, we isolated total RNA from regions of interest from human OA (n = 20) and non-OA (n = 5) knee lateral tibial (LT) and medial tibial (MT) plateaus. A whole-genome profiling study was performed on an Agilent microarray platform and analyzed using Agilent GeneSpring GX11.5. Confirmatory quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis was performed on samples from 9 OA individuals to confirm differential expression of 85 genes identified by microarray. Ingenuity Pathway Analysis (IPA) was used to investigate canonical pathways and immunohistochemical staining was performed to validate protein expression levels in samples.Results: A total of 972 differentially expressed genes were identified (fold change ≥ ± 2, P ≤0.05) between LT (minimal degeneration) and MT (significant degeneration) regions from OA samples; these data implicated 279 canonical pathways in IPA. The qRT-PCR data strongly confirmed the accuracy of microarray results (R2 = 0.58, P

Original languageEnglish
Article numberR190
JournalArthritis Research and Therapy
Volume15
Issue number6
DOIs
Publication statusPublished - Nov 15 2013

ASJC Scopus subject areas

  • Rheumatology
  • Immunology
  • Immunology and Allergy
  • Medicine(all)

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    Chou, C. H., Wu, C. C., Song, I. W., Chuang, H. P., Lu, L. S., Chang, J. H., Kuo, S. Y., Lee, C. H., Wu, J. Y., Chen, Y. T., Kraus, V. B., & Lee, M. T. M. (2013). Genome-wide expression profiles of subchondral bone in osteoarthritis. Arthritis Research and Therapy, 15(6), [R190]. https://doi.org/10.1186/ar4380