Genistein suppresses the isoproterenol-treated H9c2 cardiomyoblast cell apoptosis associated with P-38, Erk1/2, JNK, and NFκB signaling protein activation

Wei Syun Hu, Yueh Min Lin, Tsung Jung Ho, Ray Jade Chen, Yi Hui Li, Fuu Jen Tsai, Chang Hai Tsai, Cecilia Hsuan Day, Tung Sheng Chen, Chih Yang Huang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Heart disease (HD) is associated with estrogen and therefore gender and menopausal status. In addition, clinical evidence shows that increased serum norepinephrine is found in patients with HD. Therefore, this study aimed to investigate the cardio-protective effect of genistein, a selective estrogen receptor modulator (SERM) from soy bean extract, in H9c2 cardiomyoblast cells treated with isoproterenol (ISO), a norepinephrine analog. In this in vitro model, image data and results from western blotting shown that ISO treatment was capable of inducing cellular apoptosis, especially the mitochondrial dependent pathway. Treatment of genistein could suppress the expression of mitochondrial pro-apoptotic proteins including Bad, caspase-8, caspase-9, and caspase-3 in H9c2 treated with ISO. By contrast, several survival proteins were expressed in H9c2 treated with genistein, such as phosphor (p)-Akt, p-Bad, and p-Erk1/2. Furthermore, we confirmed that the protective role of genistein was partially mediated through the expression of Erk1/2, Akt, and NFκB proteins by adding several pathway inhibitors. These in vitro data suggest that genistein may be a safe and natural SERM alternative to hormone therapy in cardio-protection.

Original languageEnglish
Pages (from-to)1125-1136
Number of pages12
JournalAmerican Journal of Chinese Medicine
Volume41
Issue number5
DOIs
Publication statusPublished - 2013

Keywords

  • Estrogen
  • Genistein
  • Heart Disease
  • Isoproterenol

ASJC Scopus subject areas

  • Complementary and alternative medicine

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