Genetic variation of Anoectochilus formosanus revealed by ISSR and AFLP analysis

Shun Fu Lin, Hsin Sheng Tsay, Tsui Wei Chou, Ming Jing Yang, Kur Ta Cheng

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Anoectochilus formosanus is an expensive medicinal folk herb in Taiwan. The plant has been used to treat hypertension, diabetes, and hepatitis by native people for a long time. In this study, 4 typical lines of A. formosanus, red-stem, green-stem, narrow-leaf, and round-leaf, were collected and screened to identify the line-specific SSR (single sequence repeat) markers. In addition, we found the narrow-leaf line exhibited the highest polysaccharide content (3.39 ± 0.74%, polysaccharide wt/plant dried wt) which is more than twofold of the lowest content of the red-stem line (1.27 + 0.41%). In the culture program construction, the extent of the somaclonal variation among 20 regenerated shoots from the same parental individual of a narrow-leaf line was revealed using the amplified fragment length polymorphism (AFLP) technique. The aim of this study is to preliminarily investigate the genetic variation of A. formosanus through polymorphic marker screening, which will benefit to the cultivation of A. formosanus with high polysaccharide content in future work. The results showed that 6 out of 81 primers screened, generating a total of 26 markers, were able to differentiate the 4 lines in the inter single sequence repeat (ISSR) analysis. On the other hand, 17 sets of AFLP primers were chosen to detect the somatic variation among the excised shoots derived from the same individual. The genetic variation among the regenerated shoots ranged from 0.00% to 5.43%. The primer pair B6 exerts the most powerful selection ability in the AFLP analysis among 20 somaclonal samples used, whereas the E4 primer pair the lowest. The AFLP primer pairs become useful tools for selecting the lowest somaclonal variation from the regenerated shoots of A. formosanus under the tissue culture program.

Original languageEnglish
Pages (from-to)156-162
Number of pages7
JournalJournal of Food and Drug Analysis
Volume15
Issue number2
Publication statusPublished - Jun 2007

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Amplified Fragment Length Polymorphism Analysis
amplified fragment length polymorphism
Polysaccharides
microsatellite repeats
genetic variation
somaclonal variation
shoots
polysaccharides
stems
Medicinal Plants
leaves
Taiwan
Population Groups
Hepatitis
Sequence Analysis
clonal variation
indigenous peoples
Hypertension
hepatitis
hypertension

Keywords

  • AFLP
  • Anoectochilus formosanus
  • ISSR
  • Orchidaceae
  • Somaclonal variation

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

Genetic variation of Anoectochilus formosanus revealed by ISSR and AFLP analysis. / Lin, Shun Fu; Tsay, Hsin Sheng; Chou, Tsui Wei; Yang, Ming Jing; Cheng, Kur Ta.

In: Journal of Food and Drug Analysis, Vol. 15, No. 2, 06.2007, p. 156-162.

Research output: Contribution to journalArticle

Lin, Shun Fu ; Tsay, Hsin Sheng ; Chou, Tsui Wei ; Yang, Ming Jing ; Cheng, Kur Ta. / Genetic variation of Anoectochilus formosanus revealed by ISSR and AFLP analysis. In: Journal of Food and Drug Analysis. 2007 ; Vol. 15, No. 2. pp. 156-162.
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abstract = "Anoectochilus formosanus is an expensive medicinal folk herb in Taiwan. The plant has been used to treat hypertension, diabetes, and hepatitis by native people for a long time. In this study, 4 typical lines of A. formosanus, red-stem, green-stem, narrow-leaf, and round-leaf, were collected and screened to identify the line-specific SSR (single sequence repeat) markers. In addition, we found the narrow-leaf line exhibited the highest polysaccharide content (3.39 ± 0.74{\%}, polysaccharide wt/plant dried wt) which is more than twofold of the lowest content of the red-stem line (1.27 + 0.41{\%}). In the culture program construction, the extent of the somaclonal variation among 20 regenerated shoots from the same parental individual of a narrow-leaf line was revealed using the amplified fragment length polymorphism (AFLP) technique. The aim of this study is to preliminarily investigate the genetic variation of A. formosanus through polymorphic marker screening, which will benefit to the cultivation of A. formosanus with high polysaccharide content in future work. The results showed that 6 out of 81 primers screened, generating a total of 26 markers, were able to differentiate the 4 lines in the inter single sequence repeat (ISSR) analysis. On the other hand, 17 sets of AFLP primers were chosen to detect the somatic variation among the excised shoots derived from the same individual. The genetic variation among the regenerated shoots ranged from 0.00{\%} to 5.43{\%}. The primer pair B6 exerts the most powerful selection ability in the AFLP analysis among 20 somaclonal samples used, whereas the E4 primer pair the lowest. The AFLP primer pairs become useful tools for selecting the lowest somaclonal variation from the regenerated shoots of A. formosanus under the tissue culture program.",
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