Genetic polymorphisms of N-acetyltransferase 1 and 2 and risk of cigarette smoking-related bladder cancer

F. I. Hsieh, Y. S. Pu, H. D. Chern, L. I. Hsu, H. Y. Chiou, C. J. Chen

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Aromatic amines from cigarette smoking or occupational exposure, recognized risk factors for bladder cancer, are metabolized by N-acetyltransferases (NAT). This study examined the association of (NAT) 1 and 2 genotypes with the risk of smoking-related bladder cancer. A total of 74 pathoiogically confirmed bladder cancer patients and 184 controls were serially recruited from the National Taiwan University Hospital. History of cigarette smoking and other risk factors for bladder cancer was obtained through standardized questionnaire interview. Peripheral blood lymphocytes were collected from each subject and genotyped for NAT1 and NAT2 by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism methods. Allele frequency distributions of NAT1 and NAT2 were similar between cases and controls. There was a significant dose-response relationship between the risk of bladder cancer and the quantity and duration of cigarette smoking. The biological gradients were significant among subjects carrying NAT1*10 allele or NAT2 slow acetylators, but not among NAT2 rapid acetylators without NAT1*10 allele. The results are consistent with the hypothesis that NAT1 and NAT2 might modulate the susceptibility to bladder cancer associated with cigarette smoking.

Original languageEnglish
Pages (from-to)537-541
Number of pages5
JournalBritish Journal of Cancer
Volume81
Issue number3
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Genetic Polymorphisms
Urinary Bladder Neoplasms
Smoking
Alleles
Acetyltransferases
DNA-Directed DNA Polymerase
Occupational Exposure
Taiwan
DNA Sequence Analysis
Gene Frequency
Restriction Fragment Length Polymorphisms
Amines
N-acetyltransferase 1
Genotype
Lymphocytes
Interviews
Polymerase Chain Reaction

Keywords

  • Bladder cancer
  • Cigarette smoking
  • N-acetyltransferase 1
  • N-acetyltransferase 2

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Genetic polymorphisms of N-acetyltransferase 1 and 2 and risk of cigarette smoking-related bladder cancer. / Hsieh, F. I.; Pu, Y. S.; Chern, H. D.; Hsu, L. I.; Chiou, H. Y.; Chen, C. J.

In: British Journal of Cancer, Vol. 81, No. 3, 1999, p. 537-541.

Research output: Contribution to journalArticle

@article{b3a466c14bd64d8aa886682da9286711,
title = "Genetic polymorphisms of N-acetyltransferase 1 and 2 and risk of cigarette smoking-related bladder cancer",
abstract = "Aromatic amines from cigarette smoking or occupational exposure, recognized risk factors for bladder cancer, are metabolized by N-acetyltransferases (NAT). This study examined the association of (NAT) 1 and 2 genotypes with the risk of smoking-related bladder cancer. A total of 74 pathoiogically confirmed bladder cancer patients and 184 controls were serially recruited from the National Taiwan University Hospital. History of cigarette smoking and other risk factors for bladder cancer was obtained through standardized questionnaire interview. Peripheral blood lymphocytes were collected from each subject and genotyped for NAT1 and NAT2 by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism methods. Allele frequency distributions of NAT1 and NAT2 were similar between cases and controls. There was a significant dose-response relationship between the risk of bladder cancer and the quantity and duration of cigarette smoking. The biological gradients were significant among subjects carrying NAT1*10 allele or NAT2 slow acetylators, but not among NAT2 rapid acetylators without NAT1*10 allele. The results are consistent with the hypothesis that NAT1 and NAT2 might modulate the susceptibility to bladder cancer associated with cigarette smoking.",
keywords = "Bladder cancer, Cigarette smoking, N-acetyltransferase 1, N-acetyltransferase 2",
author = "Hsieh, {F. I.} and Pu, {Y. S.} and Chern, {H. D.} and Hsu, {L. I.} and Chiou, {H. Y.} and Chen, {C. J.}",
year = "1999",
doi = "10.1038/sj.bjc.6690727",
language = "English",
volume = "81",
pages = "537--541",
journal = "British Journal of Cancer",
issn = "0007-0920",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Genetic polymorphisms of N-acetyltransferase 1 and 2 and risk of cigarette smoking-related bladder cancer

AU - Hsieh, F. I.

AU - Pu, Y. S.

AU - Chern, H. D.

AU - Hsu, L. I.

AU - Chiou, H. Y.

AU - Chen, C. J.

PY - 1999

Y1 - 1999

N2 - Aromatic amines from cigarette smoking or occupational exposure, recognized risk factors for bladder cancer, are metabolized by N-acetyltransferases (NAT). This study examined the association of (NAT) 1 and 2 genotypes with the risk of smoking-related bladder cancer. A total of 74 pathoiogically confirmed bladder cancer patients and 184 controls were serially recruited from the National Taiwan University Hospital. History of cigarette smoking and other risk factors for bladder cancer was obtained through standardized questionnaire interview. Peripheral blood lymphocytes were collected from each subject and genotyped for NAT1 and NAT2 by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism methods. Allele frequency distributions of NAT1 and NAT2 were similar between cases and controls. There was a significant dose-response relationship between the risk of bladder cancer and the quantity and duration of cigarette smoking. The biological gradients were significant among subjects carrying NAT1*10 allele or NAT2 slow acetylators, but not among NAT2 rapid acetylators without NAT1*10 allele. The results are consistent with the hypothesis that NAT1 and NAT2 might modulate the susceptibility to bladder cancer associated with cigarette smoking.

AB - Aromatic amines from cigarette smoking or occupational exposure, recognized risk factors for bladder cancer, are metabolized by N-acetyltransferases (NAT). This study examined the association of (NAT) 1 and 2 genotypes with the risk of smoking-related bladder cancer. A total of 74 pathoiogically confirmed bladder cancer patients and 184 controls were serially recruited from the National Taiwan University Hospital. History of cigarette smoking and other risk factors for bladder cancer was obtained through standardized questionnaire interview. Peripheral blood lymphocytes were collected from each subject and genotyped for NAT1 and NAT2 by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism methods. Allele frequency distributions of NAT1 and NAT2 were similar between cases and controls. There was a significant dose-response relationship between the risk of bladder cancer and the quantity and duration of cigarette smoking. The biological gradients were significant among subjects carrying NAT1*10 allele or NAT2 slow acetylators, but not among NAT2 rapid acetylators without NAT1*10 allele. The results are consistent with the hypothesis that NAT1 and NAT2 might modulate the susceptibility to bladder cancer associated with cigarette smoking.

KW - Bladder cancer

KW - Cigarette smoking

KW - N-acetyltransferase 1

KW - N-acetyltransferase 2

UR - http://www.scopus.com/inward/record.url?scp=0032837276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032837276&partnerID=8YFLogxK

U2 - 10.1038/sj.bjc.6690727

DO - 10.1038/sj.bjc.6690727

M3 - Article

C2 - 10507782

AN - SCOPUS:0032837276

VL - 81

SP - 537

EP - 541

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 3

ER -