Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells

Hsi Chang, Momoko Yoshimoto, Katsutsugu Umeda, Toru Iwasa, Yuta Mizuno, So Ichiro Fukada, Hiroshi Yamamoto, Norio Motohashi, [No Value] Yuko-Miyagoe-Suzuki, Shin'ichi Takeda, Toshio Heike, Tatsutoshi Nakahata

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Satellite cells are myogenic stem cells responsible for the postnatal regeneration of skeletal muscle. Here we report the successful in vitro induction of Pax7-positive satellite-like cells from mouse embryonic stem (mES) cells. Embryoid bodies were generated from mES cells and cultured on Matrigel-coated dishes with Dulbecco's modified Eagle medium containing fetal bovine serum and horse serum. Pax7-positive satellite-like cells were enriched by fluorescence-activated cell sorting using a novel anti-satellite cell antibody, SM/C-2.6. SM/C-2.6-positive cells efficiently differentiate into skeletal muscle fibers both in vitro and in vivo. Furthermore, the cells demonstrate satellite cell characteristics such as extensive self-renewal capacity in subsequent muscle injury model, long-term engraftment up to 24 wk, and the ability to be secondarily transplanted with remarkably high engraftment efficiency compared to myoblast transplantation. This is the first report of transplantable, functional satellite-like cells derived from mES cells and will provide a foundation for new therapies for degenerative muscle disorders.

Original languageEnglish
Pages (from-to)1907-1919
Number of pages13
JournalFASEB Journal
Volume23
Issue number6
DOIs
Publication statusPublished - Jun 2009
Externally publishedYes

Fingerprint

Stem cells
Satellites
Muscle
Cells
Skeletal Muscle Satellite Cells
Embryoid Bodies
Eagles
Sorting
Myoblasts
Skeletal Muscle Fibers
Muscular Diseases
Mouse Embryonic Stem Cells
Serum
Horses
Fluorescence
Regeneration
Flow Cytometry
Skeletal Muscle
Stem Cells
Transplantation

Keywords

  • High engraftment efficiency
  • Long-term engraftment
  • Secondary transplantation
  • Self-renewal

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

Chang, H., Yoshimoto, M., Umeda, K., Iwasa, T., Mizuno, Y., Fukada, S. I., ... Nakahata, T. (2009). Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells. FASEB Journal, 23(6), 1907-1919. https://doi.org/10.1096/fj.08-123661

Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells. / Chang, Hsi; Yoshimoto, Momoko; Umeda, Katsutsugu; Iwasa, Toru; Mizuno, Yuta; Fukada, So Ichiro; Yamamoto, Hiroshi; Motohashi, Norio; Yuko-Miyagoe-Suzuki, [No Value]; Takeda, Shin'ichi; Heike, Toshio; Nakahata, Tatsutoshi.

In: FASEB Journal, Vol. 23, No. 6, 06.2009, p. 1907-1919.

Research output: Contribution to journalArticle

Chang, H, Yoshimoto, M, Umeda, K, Iwasa, T, Mizuno, Y, Fukada, SI, Yamamoto, H, Motohashi, N, Yuko-Miyagoe-Suzuki, NV, Takeda, S, Heike, T & Nakahata, T 2009, 'Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells', FASEB Journal, vol. 23, no. 6, pp. 1907-1919. https://doi.org/10.1096/fj.08-123661
Chang, Hsi ; Yoshimoto, Momoko ; Umeda, Katsutsugu ; Iwasa, Toru ; Mizuno, Yuta ; Fukada, So Ichiro ; Yamamoto, Hiroshi ; Motohashi, Norio ; Yuko-Miyagoe-Suzuki, [No Value] ; Takeda, Shin'ichi ; Heike, Toshio ; Nakahata, Tatsutoshi. / Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells. In: FASEB Journal. 2009 ; Vol. 23, No. 6. pp. 1907-1919.
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