Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells

Yuta Mizuno, Hsi Chang, Katsutsugu Umeda, Akira Niwa, Toru Iwasa, Tomonari Awaya, So Ichiro Fukada, Hiroshi Yamamoto, Shinya Yamanaka, Tatsutoshi Nakahata, Toshio Heike

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Induced pluripotent stem (iPS) cells, which are a type of pluripotent stem cell generated from reprogrammed somatic cells, are expected to have potential for patient-oriented disease investigation, drug screening, toxicity tests, and transplantation therapies. Here, we demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells. Cells with strong in vitro myogenic potential effectively were enriched by fluorescence-activated cell sorting using the anti-satellite cell antibody SM/C-2.6. Furthermore, on transplantation into mdx mice, SM/C-2.6+ cells exerted sustained myogenic lineage differentiation in injured muscles, while providing long-lived muscle stem cell support. Our data suggest that iPS cells have the potential to be used in clinical treatment of muscular dystrophies.

Original languageEnglish
Pages (from-to)2245-2253
Number of pages9
JournalFASEB Journal
Volume24
Issue number7
DOIs
Publication statusPublished - Jul 2010
Externally publishedYes

Keywords

  • Duchenne muscular dystrophy
  • High engraftment efficiency
  • Long-term engraftment
  • No teratoma formation
  • Pax7

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

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  • Cite this

    Mizuno, Y., Chang, H., Umeda, K., Niwa, A., Iwasa, T., Awaya, T., Fukada, S. I., Yamamoto, H., Yamanaka, S., Nakahata, T., & Heike, T. (2010). Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells. FASEB Journal, 24(7), 2245-2253. https://doi.org/10.1096/fj.09-137174