Generation of induced pluripotent stem (iPS) cells derived from a murine model of Pompe disease and differentiation of Pompe-iPS cells into skeletal muscle cells

Shiho Kawagoe, Takashi Higuchi, Xing Li Meng, Yohta Shimada, Hiromi Shimizu, Reimi Hirayama, Takahiro Fukuda, Hsi Chang, Tatsutoshi Nakahata, So Ichiro Fukada, Hiroyuki Ida, Hiroshi Kobayashi, Toya Ohashi, Yoshikastu Eto

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Our study is the first to demonstrate the ability to generate iPS cells from a mouse model of Pompe disease. Initially, mouse tail tip fibroblasts were harvested from male, 8-week-old (GAA) knockout mice, and three reprogramming factors (Oct3/4, Sox2 and Klf4) were transfected into the isolated donor cells using a retroviral vector. These iPS cells also showed decreased levels of GAA enzymatic activity and strong positive staining with periodic acid-Schiff (indicating the accumulation of glycogen) and acid phosphatase (lysosomal activation marker). Pompe-iPS cells were differentiated into skeletal muscle cells in Matrigel®-coated plates. Spindle-shaped skeletal muscle cells were successfully generated from Pompe-iPS cells and showed spontaneous contraction and positive staining with the myosin heavy chain antibody. Electron microscopic analysis of the skeletal muscle cells showed typical morphological features, including Z-bands, I-bands, A-bands and H-bands, which were visible in wild-type and Pompe cells. Furthermore, Pompe skeletal muscle cells accumulated massive glycogen in lysosomes. This study indicates that the iPS and skeletal muscle cells generated in this study could also be a useful disease model for studies investigating the pathogenesis and treatment of skeletal muscle in Pompe disease.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalMolecular Genetics and Metabolism
Volume104
Issue number1-2
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Glycogen Storage Disease Type II
Induced Pluripotent Stem Cells
Stem cells
Muscle Cells
Muscle
Skeletal Muscle
Cells
Glycogen
Staining and Labeling
Periodic Acid
Myosin Heavy Chains
Fibroblasts
Acid Phosphatase
Lysosomes
Knockout Mice
Tail
Chemical activation
Electrons
Antibodies

Keywords

  • Acid α-glucosidase
  • Induced pluripotent stem (iPS)
  • Pompe disease
  • Reprogramming factor
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Generation of induced pluripotent stem (iPS) cells derived from a murine model of Pompe disease and differentiation of Pompe-iPS cells into skeletal muscle cells. / Kawagoe, Shiho; Higuchi, Takashi; Meng, Xing Li; Shimada, Yohta; Shimizu, Hiromi; Hirayama, Reimi; Fukuda, Takahiro; Chang, Hsi; Nakahata, Tatsutoshi; Fukada, So Ichiro; Ida, Hiroyuki; Kobayashi, Hiroshi; Ohashi, Toya; Eto, Yoshikastu.

In: Molecular Genetics and Metabolism, Vol. 104, No. 1-2, 09.2011, p. 123-128.

Research output: Contribution to journalArticle

Kawagoe, S, Higuchi, T, Meng, XL, Shimada, Y, Shimizu, H, Hirayama, R, Fukuda, T, Chang, H, Nakahata, T, Fukada, SI, Ida, H, Kobayashi, H, Ohashi, T & Eto, Y 2011, 'Generation of induced pluripotent stem (iPS) cells derived from a murine model of Pompe disease and differentiation of Pompe-iPS cells into skeletal muscle cells', Molecular Genetics and Metabolism, vol. 104, no. 1-2, pp. 123-128. https://doi.org/10.1016/j.ymgme.2011.05.020
Kawagoe, Shiho ; Higuchi, Takashi ; Meng, Xing Li ; Shimada, Yohta ; Shimizu, Hiromi ; Hirayama, Reimi ; Fukuda, Takahiro ; Chang, Hsi ; Nakahata, Tatsutoshi ; Fukada, So Ichiro ; Ida, Hiroyuki ; Kobayashi, Hiroshi ; Ohashi, Toya ; Eto, Yoshikastu. / Generation of induced pluripotent stem (iPS) cells derived from a murine model of Pompe disease and differentiation of Pompe-iPS cells into skeletal muscle cells. In: Molecular Genetics and Metabolism. 2011 ; Vol. 104, No. 1-2. pp. 123-128.
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