Generation and characterization of androgen receptor knockout (ARKO) mice

An in vivo model for the study of androgen functions in selective tissues

Shuyuan Yeh, Meng Yin Tsai, Qingquan Xu, Xiao Min Mu, Henry Lardy, Ko En Huang, Hank Lin, Shauh Der Yeh, Saleh Altuwaijri, Xinchang Zhou, Lianping Xing, Brendan F. Boyce, Mien Chie Hung, Su Zhang, Lin Gan, Chawnshang Chang

Research output: Contribution to journalArticle

420 Citations (Scopus)

Abstract

By using a cre-lox conditional knockout strategy, we report here the generation of androgen receptor knockout (ARKO) mice. Phenotype analysis shows that ARKO male mice have a female-like appearance and body weight. Their testes are 80% smaller and serum testosterone concentrations are lower than in wild-type (wt) mice. Spermatogenesis is arrested at pachytene spermatocytes. The number and size of adipocytes are also different between the wt and ARKO mice. Cancellous bone volumes of ARKO male mice are reduced compared with wt littermates. In addition, we found the average number of pups per litter in homologous and heterozygous ARKO female mice is lower than in wt female mice, suggesting potential defects in female fertility and/or ovulation. The cre-lox ARKO mouse provides a much-needed in vivo animal model to study androgen functions in the selective androgen target tissues in female or male mice.

Original languageEnglish
Pages (from-to)13498-13503
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number21
DOIs
Publication statusPublished - Oct 15 2002
Externally publishedYes

Fingerprint

Androgen Receptors
Knockout Mice
Androgens
Spermatocytes
Spermatogenesis
Ovulation
Adipocytes
Fertility
Testosterone
Testis
Animal Models
Body Weight
Phenotype
Serum

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Generation and characterization of androgen receptor knockout (ARKO) mice : An in vivo model for the study of androgen functions in selective tissues. / Yeh, Shuyuan; Tsai, Meng Yin; Xu, Qingquan; Mu, Xiao Min; Lardy, Henry; Huang, Ko En; Lin, Hank; Yeh, Shauh Der; Altuwaijri, Saleh; Zhou, Xinchang; Xing, Lianping; Boyce, Brendan F.; Hung, Mien Chie; Zhang, Su; Gan, Lin; Chang, Chawnshang.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 21, 15.10.2002, p. 13498-13503.

Research output: Contribution to journalArticle

Yeh, S, Tsai, MY, Xu, Q, Mu, XM, Lardy, H, Huang, KE, Lin, H, Yeh, SD, Altuwaijri, S, Zhou, X, Xing, L, Boyce, BF, Hung, MC, Zhang, S, Gan, L & Chang, C 2002, 'Generation and characterization of androgen receptor knockout (ARKO) mice: An in vivo model for the study of androgen functions in selective tissues', Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 21, pp. 13498-13503. https://doi.org/10.1073/pnas.212474399
Yeh, Shuyuan ; Tsai, Meng Yin ; Xu, Qingquan ; Mu, Xiao Min ; Lardy, Henry ; Huang, Ko En ; Lin, Hank ; Yeh, Shauh Der ; Altuwaijri, Saleh ; Zhou, Xinchang ; Xing, Lianping ; Boyce, Brendan F. ; Hung, Mien Chie ; Zhang, Su ; Gan, Lin ; Chang, Chawnshang. / Generation and characterization of androgen receptor knockout (ARKO) mice : An in vivo model for the study of androgen functions in selective tissues. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 21. pp. 13498-13503.
@article{4cdaa7ebd93f4ac69a7be99a8638cf3a,
title = "Generation and characterization of androgen receptor knockout (ARKO) mice: An in vivo model for the study of androgen functions in selective tissues",
abstract = "By using a cre-lox conditional knockout strategy, we report here the generation of androgen receptor knockout (ARKO) mice. Phenotype analysis shows that ARKO male mice have a female-like appearance and body weight. Their testes are 80{\%} smaller and serum testosterone concentrations are lower than in wild-type (wt) mice. Spermatogenesis is arrested at pachytene spermatocytes. The number and size of adipocytes are also different between the wt and ARKO mice. Cancellous bone volumes of ARKO male mice are reduced compared with wt littermates. In addition, we found the average number of pups per litter in homologous and heterozygous ARKO female mice is lower than in wt female mice, suggesting potential defects in female fertility and/or ovulation. The cre-lox ARKO mouse provides a much-needed in vivo animal model to study androgen functions in the selective androgen target tissues in female or male mice.",
author = "Shuyuan Yeh and Tsai, {Meng Yin} and Qingquan Xu and Mu, {Xiao Min} and Henry Lardy and Huang, {Ko En} and Hank Lin and Yeh, {Shauh Der} and Saleh Altuwaijri and Xinchang Zhou and Lianping Xing and Boyce, {Brendan F.} and Hung, {Mien Chie} and Su Zhang and Lin Gan and Chawnshang Chang",
year = "2002",
month = "10",
day = "15",
doi = "10.1073/pnas.212474399",
language = "English",
volume = "99",
pages = "13498--13503",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "21",

}

TY - JOUR

T1 - Generation and characterization of androgen receptor knockout (ARKO) mice

T2 - An in vivo model for the study of androgen functions in selective tissues

AU - Yeh, Shuyuan

AU - Tsai, Meng Yin

AU - Xu, Qingquan

AU - Mu, Xiao Min

AU - Lardy, Henry

AU - Huang, Ko En

AU - Lin, Hank

AU - Yeh, Shauh Der

AU - Altuwaijri, Saleh

AU - Zhou, Xinchang

AU - Xing, Lianping

AU - Boyce, Brendan F.

AU - Hung, Mien Chie

AU - Zhang, Su

AU - Gan, Lin

AU - Chang, Chawnshang

PY - 2002/10/15

Y1 - 2002/10/15

N2 - By using a cre-lox conditional knockout strategy, we report here the generation of androgen receptor knockout (ARKO) mice. Phenotype analysis shows that ARKO male mice have a female-like appearance and body weight. Their testes are 80% smaller and serum testosterone concentrations are lower than in wild-type (wt) mice. Spermatogenesis is arrested at pachytene spermatocytes. The number and size of adipocytes are also different between the wt and ARKO mice. Cancellous bone volumes of ARKO male mice are reduced compared with wt littermates. In addition, we found the average number of pups per litter in homologous and heterozygous ARKO female mice is lower than in wt female mice, suggesting potential defects in female fertility and/or ovulation. The cre-lox ARKO mouse provides a much-needed in vivo animal model to study androgen functions in the selective androgen target tissues in female or male mice.

AB - By using a cre-lox conditional knockout strategy, we report here the generation of androgen receptor knockout (ARKO) mice. Phenotype analysis shows that ARKO male mice have a female-like appearance and body weight. Their testes are 80% smaller and serum testosterone concentrations are lower than in wild-type (wt) mice. Spermatogenesis is arrested at pachytene spermatocytes. The number and size of adipocytes are also different between the wt and ARKO mice. Cancellous bone volumes of ARKO male mice are reduced compared with wt littermates. In addition, we found the average number of pups per litter in homologous and heterozygous ARKO female mice is lower than in wt female mice, suggesting potential defects in female fertility and/or ovulation. The cre-lox ARKO mouse provides a much-needed in vivo animal model to study androgen functions in the selective androgen target tissues in female or male mice.

UR - http://www.scopus.com/inward/record.url?scp=0037108766&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037108766&partnerID=8YFLogxK

U2 - 10.1073/pnas.212474399

DO - 10.1073/pnas.212474399

M3 - Article

VL - 99

SP - 13498

EP - 13503

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 21

ER -