Gene-to-gene network analysis of the mediation of plant innate immunity by the eliciting plant response-like 1 (Epl1) elicitor of trichoderma formosa

Chi Hua Cheng, Bing Nan Shen, Qian Wen Shang, Li Yu Daisy Liu, Kou Cheng Peng, Yan Huey Chen, Fang Fang Chen, Sin Fen Hu, Yu Tai Wang, Hao Ching Wang, Hsin Yi Wu, Chaur Tsuen Lo, Shih Shun Lin

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4 Citations (Scopus)

Abstract

A new clade, Trichoderma formosa, secretes eliciting plant response-like 1 (Epl1), a small peptide elicitor that stimulates plant immunity. Nicotiana benthamiana pretreated with Epl1 for 3 days developed immunity against Tomato mosaic virus (ToMV) infection. The transcriptome profiles of T. formosa and N. benthamiana were obtained by deep sequencing; the transcript of Epl1 is 736 nt in length and encodes a 12-kDa peptide. Identifying critical genes in Epl1-mediated immunity was challenging due to high similarity between the transcriptome expression profiles of Epl1-treated and ToMV-infected N. benthamiana samples. Therefore, an efficient bioinformatics data mining approach was used for high-throughput transcriptomic assays in this study. We integrated gene-to-gene network analysis into the ContigViews transcriptome database, and genes related to jasmonic acid and ethylene signaling, salicylic acid signaling, leucine-rich repeats, transcription factors, and histone variants were hubs in the gene-to-gene networks. In this study, the Epl1 of T. formosa triggers plant immunity against various pathogen infections. Moreover, we demonstrated that high-throughput data mining and gene-to-gene network analysis can be used to identify critical candidate genes for further studies on the mechanisms of plant immunity.

Original languageEnglish
Pages (from-to)683-691
Number of pages9
JournalMolecular Plant-Microbe Interactions
Volume31
Issue number7
DOIs
Publication statusPublished - Jul 1 2018

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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  • Cite this

    Cheng, C. H., Shen, B. N., Shang, Q. W., Liu, L. Y. D., Peng, K. C., Chen, Y. H., Chen, F. F., Hu, S. F., Wang, Y. T., Wang, H. C., Wu, H. Y., Lo, C. T., & Lin, S. S. (2018). Gene-to-gene network analysis of the mediation of plant innate immunity by the eliciting plant response-like 1 (Epl1) elicitor of trichoderma formosa. Molecular Plant-Microbe Interactions, 31(7), 683-691. https://doi.org/10.1094/MPMI-01-18-0002-TA