Abstract
Chronic kidney disease (CKD) is highly prevalent in Taiwan and an increasing number of patients are affected, with a high risk of progression to end-stage renal disease and huge medical expenses. It has been predicted that the presence of hypertension increases with decreasing renal function due to a decrease in sodium excretion and activation of the renin-angiotensin system (RAS). The aim of this study was to investigate the influence of genetic variants of the RAS gene on CKD.We performed a case control association study and genotyped 135 CKD patients and 270 healthy controls among Han Chinese in Taiwan. All subjects were genotyped for angiotensinogen (AGT-M235T, T174M, A-20C), angiotensin-I converting enzyme (ACE-A2350G) and angiotensin II type 1 receptor (AGTR1-A1166C, C573T, C-521T) polymorphisms of RAS genes by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Significant associations were observed in ACE-A2350G and AGTR1-C573T polymorphism between CKD patients and controls. In regard to ACE-A2350G, compared with the AA genotype the GG genotype protected against CKD (adjusted odds ratio [OR] = 0.34; p = 0.01). In regard to AGTR1-C573T, the CT genotype was a risk for CKD compared with the CC genotype (adjusted OR = 1.82; p = 0.03). We conclude that ACE-A2350G and AGTR1-C573T polymorphisms are likely candidate determinants of CKD.
| Original language | English |
|---|---|
| Pages (from-to) | 148-154 |
| Number of pages | 7 |
| Journal | JRAAS - Journal of the Renin-Angiotensin-Aldosterone System |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 2012 |
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Keywords
- angiotensin II type 1 receptor (AGTR1)
- angiotensin-I converting enzyme (ACE)
- angiotensinogen (AGT)
- Chronic kidney disease (CKD)
- single nucleotide polymorphism (SNP)
ASJC Scopus subject areas
- Internal Medicine
- Endocrinology
Cite this
Gene polymorphisms of angiotensin-converting enzyme and angiotensin II Type I receptor among chronic kidney disease patients in a Chinese population. / Su, Sui Lung; Lu, Kuo Cheng; Lin, Yuh Feng; Hsu, Yu Juei; Lee, Pong Ying; Yang, Hsin Yi; Kao, Sen Yeong.
In: JRAAS - Journal of the Renin-Angiotensin-Aldosterone System, Vol. 13, No. 1, 03.2012, p. 148-154.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gene polymorphisms of angiotensin-converting enzyme and angiotensin II Type I receptor among chronic kidney disease patients in a Chinese population
AU - Su, Sui Lung
AU - Lu, Kuo Cheng
AU - Lin, Yuh Feng
AU - Hsu, Yu Juei
AU - Lee, Pong Ying
AU - Yang, Hsin Yi
AU - Kao, Sen Yeong
PY - 2012/3
Y1 - 2012/3
N2 - Chronic kidney disease (CKD) is highly prevalent in Taiwan and an increasing number of patients are affected, with a high risk of progression to end-stage renal disease and huge medical expenses. It has been predicted that the presence of hypertension increases with decreasing renal function due to a decrease in sodium excretion and activation of the renin-angiotensin system (RAS). The aim of this study was to investigate the influence of genetic variants of the RAS gene on CKD.We performed a case control association study and genotyped 135 CKD patients and 270 healthy controls among Han Chinese in Taiwan. All subjects were genotyped for angiotensinogen (AGT-M235T, T174M, A-20C), angiotensin-I converting enzyme (ACE-A2350G) and angiotensin II type 1 receptor (AGTR1-A1166C, C573T, C-521T) polymorphisms of RAS genes by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Significant associations were observed in ACE-A2350G and AGTR1-C573T polymorphism between CKD patients and controls. In regard to ACE-A2350G, compared with the AA genotype the GG genotype protected against CKD (adjusted odds ratio [OR] = 0.34; p = 0.01). In regard to AGTR1-C573T, the CT genotype was a risk for CKD compared with the CC genotype (adjusted OR = 1.82; p = 0.03). We conclude that ACE-A2350G and AGTR1-C573T polymorphisms are likely candidate determinants of CKD.
AB - Chronic kidney disease (CKD) is highly prevalent in Taiwan and an increasing number of patients are affected, with a high risk of progression to end-stage renal disease and huge medical expenses. It has been predicted that the presence of hypertension increases with decreasing renal function due to a decrease in sodium excretion and activation of the renin-angiotensin system (RAS). The aim of this study was to investigate the influence of genetic variants of the RAS gene on CKD.We performed a case control association study and genotyped 135 CKD patients and 270 healthy controls among Han Chinese in Taiwan. All subjects were genotyped for angiotensinogen (AGT-M235T, T174M, A-20C), angiotensin-I converting enzyme (ACE-A2350G) and angiotensin II type 1 receptor (AGTR1-A1166C, C573T, C-521T) polymorphisms of RAS genes by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Significant associations were observed in ACE-A2350G and AGTR1-C573T polymorphism between CKD patients and controls. In regard to ACE-A2350G, compared with the AA genotype the GG genotype protected against CKD (adjusted odds ratio [OR] = 0.34; p = 0.01). In regard to AGTR1-C573T, the CT genotype was a risk for CKD compared with the CC genotype (adjusted OR = 1.82; p = 0.03). We conclude that ACE-A2350G and AGTR1-C573T polymorphisms are likely candidate determinants of CKD.
KW - angiotensin II type 1 receptor (AGTR1)
KW - angiotensin-I converting enzyme (ACE)
KW - angiotensinogen (AGT)
KW - Chronic kidney disease (CKD)
KW - single nucleotide polymorphism (SNP)
UR - http://www.scopus.com/inward/record.url?scp=84859939183&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84859939183&partnerID=8YFLogxK
U2 - 10.1177/1470320311430989
DO - 10.1177/1470320311430989
M3 - Article
C2 - 22147663
AN - SCOPUS:84859939183
VL - 13
SP - 148
EP - 154
JO - JRAAS - Journal of the Renin-Angiotensin-Aldosterone System
JF - JRAAS - Journal of the Renin-Angiotensin-Aldosterone System
SN - 1470-3203
IS - 1
ER -