Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide

Nobuyuki Yamamichi, Kamolparn Pugdee, Wei Jen Chang, Sheng Yang Lee, Masao Yoshinari, Tohru Hayakawa, Yoshimitsu Abiko

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Enhanced adhesion and migration of osteoblastic cells on titanium (Ti) surface is believed to increase the success rate of implant therapy. A GRGDSP peptide derived from fibronectin was coated on Ti surfaces using a tresyl chloride activation technique, and then MC3T3-E1 osteoblastic cells were cultured on the Ti surfaces. After 15 days, total RNA was isolated from the cells and gene expression level were analyzed by Affymetrix GeneChip system. The expression levels of many genes in MC3T3-E1 cells cultured on GRGDSP-coated Ti surface were altered when compared to uncoated Ti. In particular, the elevated mRNA levels of bone sialoprotein (BSP) and osteocalcin (OC) were successfully confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. In light of the results obtained, GRGDSP-coated Ti presented the potential of evolving into a useful biomaterial for successful implant therapy.

Original languageEnglish
Pages (from-to)744-750
Number of pages7
JournalDental Materials Journal
Volume27
Issue number5
DOIs
Publication statusPublished - 2008

Fingerprint

Osteoblasts
Gene Expression Profiling
Titanium
Fibronectins
Gene expression
glycyl-arginyl-glycyl-aspartyl-seryl-proline
Peptides
Monitoring
Cultured Cells
Integrin-Binding Sialoprotein
Polymerase chain reaction
Osteocalcin
Biocompatible Materials
Transcription
RNA
Biomaterials
Reverse Transcription
Cell Movement
Real-Time Polymerase Chain Reaction
Bone

Keywords

  • Genechip
  • Osteoblast
  • RGD peptide

ASJC Scopus subject areas

  • Dentistry(all)
  • Ceramics and Composites

Cite this

Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide. / Yamamichi, Nobuyuki; Pugdee, Kamolparn; Chang, Wei Jen; Lee, Sheng Yang; Yoshinari, Masao; Hayakawa, Tohru; Abiko, Yoshimitsu.

In: Dental Materials Journal, Vol. 27, No. 5, 2008, p. 744-750.

Research output: Contribution to journalArticle

Yamamichi, Nobuyuki ; Pugdee, Kamolparn ; Chang, Wei Jen ; Lee, Sheng Yang ; Yoshinari, Masao ; Hayakawa, Tohru ; Abiko, Yoshimitsu. / Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide. In: Dental Materials Journal. 2008 ; Vol. 27, No. 5. pp. 744-750.
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