Gelatin nanoparticles as gene carriers for transgenic chicken applications

Ching Li Tseng, Chu Li Peng, Jian Yuan Huang, Jung Chih Chen, Feng Huei Lin

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To develop a safe and effective nonviral gene delivery system for transgenic chicken manipulation, we developed gelatin nanocarriers using a reporter plasmid (pEGFP-C1; enhanced green fluorescence protein, EGFP) that expressed EGFP. pEGFP-C1-containing gelatin nanoparticles (GP/pEGFP) were prepared using a water-ethanol solvent displacement method and characterized by size, surface charge, DNA loading, and DNA protection ability. For gene delivery, pEGFP-C1 was stably and efficiently encapsulated in GPs that were approximately 300 nm in diameter with a slight negative surface charge, which was prepared from gelatin solution at pH 8.0. Approximately, 85% of the plasmid DNA was encapsulated in the GPs. Electrophoresis results showed that the GPs provided protection against DNase I digestion. We used the GP/pEGFP as a vector to transfect cells and chicken embryos. The vector was nontoxic to cells, and GFP expression was effectively expressed 24 h after HeLa cell transfection. Direct injection was adapted for vector transport to the chicken embryo; injection in the area opaca (Ao) of the egg resulted in the highest hatching rate without affecting embryo development. GFP gene expression in embryo sections was observed 4 days after injection. The results of this study demonstrate that GPs are a suitable nonviral vector for delivering exogenous genes for transgenic chicken manipulation.

Original languageEnglish
Pages (from-to)1055-1065
Number of pages11
JournalJournal of Biomaterials Applications
Volume27
Issue number8
DOIs
Publication statusPublished - May 2013

Fingerprint

Gelatin
Genes
Nanoparticles
DNA
Surface charge
Plasmids
Fluorescence
Proteins
Deoxyribonuclease I
Direct injection
Electrophoresis
Gene expression
Ethanol
Water

Keywords

  • Gelatin nanoparticles
  • gene delivery
  • green fluorescence protein
  • transgenic chickens
  • vector

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Gelatin nanoparticles as gene carriers for transgenic chicken applications. / Tseng, Ching Li; Peng, Chu Li; Huang, Jian Yuan; Chen, Jung Chih; Lin, Feng Huei.

In: Journal of Biomaterials Applications, Vol. 27, No. 8, 05.2013, p. 1055-1065.

Research output: Contribution to journalArticle

Tseng, Ching Li ; Peng, Chu Li ; Huang, Jian Yuan ; Chen, Jung Chih ; Lin, Feng Huei. / Gelatin nanoparticles as gene carriers for transgenic chicken applications. In: Journal of Biomaterials Applications. 2013 ; Vol. 27, No. 8. pp. 1055-1065.
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