Abstract
Our previous studies showed that Gastrodia elata (GE), an herb used in traditional Chinese medicine, has both anti-convulsive and free radical-scavenging activities in kainic acid (KA)-treated rats. The aim of the present study was to further investigate possible physiological mechanisms of GE against activities of neuronal nitric oxide synthase (nNOS) and microglia in KA-treated rats; 0.5 g/kg and 1.0 g/kg of GE extract were administered orally, whereas 20 mg/kg of N-nitro-L-arginine methyl ester (L-NAME) was administered intraperitoneally (ip), both at 30 minutes prior to KA (2 μg/2 μl) being injected into the right hippocampus region of rats. ED1-staining, apoptotic, inducible nitric oxide synthase (iNOS), and nNOS-staining cells were observed in the hippocampus region. The results indicated that 1.0 g/kg of GE and 20 mg/kg of L-NAME reduced the counts of ED1-stained cells, and 0.5 g/kg and 1.0 g/kg of GE, and 20 mg/kg of L-NAME reduced the numbers of apoptotic cells and nNOS-staining cells. In addition, 20 mg/kg of L-NAME also reduced the numbers of iNOS-staining cells, but 0.5 g/kg and 1.0 g/kg of GE did not. This study demonstrated that GE was able to reduce nNOS, microglia activation and apoptosis, suggesting that GE has a protective effect against neuronal damage in KA-treated rats.
| Original language | English |
|---|---|
| Pages (from-to) | 599-611 |
| Number of pages | 13 |
| Journal | American Journal of Chinese Medicine |
| Volume | 33 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2005 |
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Keywords
- Apoptosis
- Gastrodia elata
- Kainic acid
- Microglia
- Nitric oxide synthase (NOS)
ASJC Scopus subject areas
- Complementary and alternative medicine
Cite this
Gastrodia elata BL mediates the suppression of nnos and microglia activation to protect against neuronal damage in kainic acid-treated rats. / Hsieh, Ching Liang; Chen, Chi Long; Tang, Nou Ying; Chuang, Chin Min; Hsieh, Ching Tou; Chiang, Su Yin; Lin, Jaung Geng; Hsu, Sheng Feng.
In: American Journal of Chinese Medicine, Vol. 33, No. 4, 2005, p. 599-611.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gastrodia elata BL mediates the suppression of nnos and microglia activation to protect against neuronal damage in kainic acid-treated rats
AU - Hsieh, Ching Liang
AU - Chen, Chi Long
AU - Tang, Nou Ying
AU - Chuang, Chin Min
AU - Hsieh, Ching Tou
AU - Chiang, Su Yin
AU - Lin, Jaung Geng
AU - Hsu, Sheng Feng
PY - 2005
Y1 - 2005
N2 - Our previous studies showed that Gastrodia elata (GE), an herb used in traditional Chinese medicine, has both anti-convulsive and free radical-scavenging activities in kainic acid (KA)-treated rats. The aim of the present study was to further investigate possible physiological mechanisms of GE against activities of neuronal nitric oxide synthase (nNOS) and microglia in KA-treated rats; 0.5 g/kg and 1.0 g/kg of GE extract were administered orally, whereas 20 mg/kg of N-nitro-L-arginine methyl ester (L-NAME) was administered intraperitoneally (ip), both at 30 minutes prior to KA (2 μg/2 μl) being injected into the right hippocampus region of rats. ED1-staining, apoptotic, inducible nitric oxide synthase (iNOS), and nNOS-staining cells were observed in the hippocampus region. The results indicated that 1.0 g/kg of GE and 20 mg/kg of L-NAME reduced the counts of ED1-stained cells, and 0.5 g/kg and 1.0 g/kg of GE, and 20 mg/kg of L-NAME reduced the numbers of apoptotic cells and nNOS-staining cells. In addition, 20 mg/kg of L-NAME also reduced the numbers of iNOS-staining cells, but 0.5 g/kg and 1.0 g/kg of GE did not. This study demonstrated that GE was able to reduce nNOS, microglia activation and apoptosis, suggesting that GE has a protective effect against neuronal damage in KA-treated rats.
AB - Our previous studies showed that Gastrodia elata (GE), an herb used in traditional Chinese medicine, has both anti-convulsive and free radical-scavenging activities in kainic acid (KA)-treated rats. The aim of the present study was to further investigate possible physiological mechanisms of GE against activities of neuronal nitric oxide synthase (nNOS) and microglia in KA-treated rats; 0.5 g/kg and 1.0 g/kg of GE extract were administered orally, whereas 20 mg/kg of N-nitro-L-arginine methyl ester (L-NAME) was administered intraperitoneally (ip), both at 30 minutes prior to KA (2 μg/2 μl) being injected into the right hippocampus region of rats. ED1-staining, apoptotic, inducible nitric oxide synthase (iNOS), and nNOS-staining cells were observed in the hippocampus region. The results indicated that 1.0 g/kg of GE and 20 mg/kg of L-NAME reduced the counts of ED1-stained cells, and 0.5 g/kg and 1.0 g/kg of GE, and 20 mg/kg of L-NAME reduced the numbers of apoptotic cells and nNOS-staining cells. In addition, 20 mg/kg of L-NAME also reduced the numbers of iNOS-staining cells, but 0.5 g/kg and 1.0 g/kg of GE did not. This study demonstrated that GE was able to reduce nNOS, microglia activation and apoptosis, suggesting that GE has a protective effect against neuronal damage in KA-treated rats.
KW - Apoptosis
KW - Gastrodia elata
KW - Kainic acid
KW - Microglia
KW - Nitric oxide synthase (NOS)
UR - http://www.scopus.com/inward/record.url?scp=23944451375&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=23944451375&partnerID=8YFLogxK
U2 - 10.1142/S0192415X0500320X
DO - 10.1142/S0192415X0500320X
M3 - Article
C2 - 16173534
AN - SCOPUS:23944451375
VL - 33
SP - 599
EP - 611
JO - American Journal of Chinese Medicine
JF - American Journal of Chinese Medicine
SN - 0192-415X
IS - 4
ER -