Gas chromatographic-mass spectrometric assay for 2-methoxyethanol and 2-methoxyacetic acid in human plasma and its application to pharmacokinetic studies

Tung Sheng Shih, Ryh Nan Pan, Jui Shu Chou, Cheng Yao Chen, Yoa Pu Hu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A new assay has been developed for simultaneous measurement of 2-methoxyethanol (ME) and its major toxic metabolite, 2-methoxyacetic acid (MAA) in human blood by gas chromatography-mass spectrometry. Over the concentration range 0.3 to 200 μg mL-1, ME and MAA could be determined with a pooled coefficient of variation (eleven concentrations, six replicate samples) of 6.5% and 8.7%, respectively. The limits of quantification of ME and MAA were 0.19 and 0.05 μg mL-1, respectively. The respective mean (SD) recoveries of ME and MAA were 101.3 (2.6)% and 98.2 (4.9)% (n = 3). A study of the effects of human contact with ME liquid showed that this new method is a specific, sensitive, simple, and reliable method both for the pharmacokinetic studies and for the biological monitoring of occupational exposure to ME.

Original languageEnglish
Pages (from-to)389-393
Number of pages5
JournalChromatographia
Volume54
Issue number5-6
Publication statusPublished - Oct 10 2001
Externally publishedYes

Fingerprint

Plasma (human)
Pharmacokinetics
Assays
Gases
Environmental Monitoring
Poisons
Occupational Exposure
Metabolites
Gas chromatography
Contacts (fluid mechanics)
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Blood
Recovery
methyl cellosolve
methoxyacetic acid
Monitoring
Liquids

Keywords

  • 2-Methoxyacetic acid
  • 2-Methoxyethanol
  • Blood samples
  • Gas chromatography - mass spectrometry
  • Occupational hygiene

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Gas chromatographic-mass spectrometric assay for 2-methoxyethanol and 2-methoxyacetic acid in human plasma and its application to pharmacokinetic studies. / Shih, Tung Sheng; Pan, Ryh Nan; Chou, Jui Shu; Chen, Cheng Yao; Hu, Yoa Pu.

In: Chromatographia, Vol. 54, No. 5-6, 10.10.2001, p. 389-393.

Research output: Contribution to journalArticle

Shih, Tung Sheng ; Pan, Ryh Nan ; Chou, Jui Shu ; Chen, Cheng Yao ; Hu, Yoa Pu. / Gas chromatographic-mass spectrometric assay for 2-methoxyethanol and 2-methoxyacetic acid in human plasma and its application to pharmacokinetic studies. In: Chromatographia. 2001 ; Vol. 54, No. 5-6. pp. 389-393.
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