@article{e14b226129e04359b00877ac901e73c8,
title = "Ganoderma lucidum polysaccharides prevent platelet-derived growth factor-stimulated smooth muscle cell proliferation in vitro and neointimal hyperplasia in the endothelial-denuded artery in vivo",
abstract = "Ganoderma lucidum is used in traditional Chinese medicine to prevent or treat a variety of diseases, including cardiovascular disorders. We previously demonstrated that a glucan-containing extract of Reishi polysaccharides (EORP) has the potent anti-inflammatory action of reducing ICAM-1 expression in lipopolysaccharide (LPS)-treated human aortic smooth muscle cells (HASMCs) and LPS-treated mice. In the present study, we examined whether EORP inhibited platelet-derived growth factor-BB (PDGF)-stimulated HASMC proliferation and the mechanism involved. EORP dose-dependently reduced cell numbers and DNA synthesis of PDGF-treated HASMCs in vitro. EORP also arrested cell cycle progression in the G0/G1 phase, and this was associated with decreased expression of cyclin D1, cyclin E, CDK2, CDK4, and p21Cip1 and upregulation of the cyclin-dependent kinase inhibitor p27Kip1. The anti-proliferative effect of EORP was partly mediated by downregulation of PDGF-induced JNK phosphorylation. In in vivo studies, the femoral artery of C57BL/6 mice was endothelial-denuded and the mice were fed a diet containing 100mg/kg/day of EORP. On day 14, both cell proliferation (proliferating cell nuclear antigen-positive cells) in the neointima and the neointima/media area ratio (0.67±0.03 vs. 1.46±0.30) were significantly reduced. Our data show that EORP interferes with the mitogenic activation of JNK, preventing entry of HASMCs into the cell cycle in vitro and reducing cell proliferation in the neointima and decreasing the neointimal area in vivo. Thus, EORP may represent a safe and effective novel approach to the prevention and treatment of vascular proliferative diseases. {\textcopyright} 2011 Wiley Periodicals, Inc.",
keywords = "cyclin D1, cyclin dependent kinase 4, cyclin dependent kinase inhibitor 1B, cyclin E, Janus kinase, plant extract, platelet derived growth factor, protein p21, reishi polysaccharide, unclassified drug, animal cell, animal experiment, animal model, animal tissue, article, cell count, cell cycle G0 phase, cell cycle G1 phase, cell cycle progression, cell proliferation, cell viability, controlled study, diet, DNA synthesis, endothelium, enzyme activation, enzyme phosphorylation, erythrocyte, femoral artery, Ganoderma lucidum, human, human cell, hyperplasia, in vitro study, in vivo study, male, mitogenesis, mouse, neointimal hyperplasia, nonhuman, priority journal, protein expression, smooth muscle fiber, upregulation, Animals, Aorta, Cell Cycle, Cell Death, Cell Proliferation, Drugs, Chinese Herbal, Gene Knockdown Techniques, Humans, Lipopolysaccharides, Male, MAP Kinase Kinase 4, Mice, Mice, Inbred C57BL, Myocytes, Smooth Muscle, Neointima, Phosphorylation, Platelet-Derived Growth Factor, Polysaccharides, Reishi, Mus",
author = "Shu-Huei Wang and Chan-Jung Liang and Yu-Wen Weng and Yung-Hsiang Chen and Hsien-Yeh Hsu and Hsiung-Fei Chien and Jaw-Shiun Tsai and Ying-Chin Tseng and Chi-Yuan Li and Yuh-Lien Chen",
note = "被引用次數：6 Export Date: 16 March 2016 CODEN: JCLLA 通訊地址: Chen, Y.-L.; Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, No. 1, Section 1, Ren-Ai Rd, Taipei 100, Taiwan; 電子郵件： ylchenv@ntu.edu.tw 化學物質/CAS: Janus kinase, 161384-16-3; cyclin dependent kinase 4, 147014-97-9; protein p21, 85306-28-1; Drugs, Chinese Herbal; Lipopolysaccharides; MAP Kinase Kinase 4, 2.7.12.2; Platelet-Derived Growth Factor; Polysaccharides 參考文獻: Andres, V., Castro, C., Antiproliferative strategies for the treatment of vascular proliferative disease (2003) Curr Vasc Pharmacol, 1, pp. 85-98; Desbois-Mouthon, C., Cadoret, A., Blivet-Van Eggelpoel, M.J., Bertrand, F., Caron, M., Atfi, A., Cherqui, G., Capeau, J., Insulin-mediated cell proliferation and survival involve inhibition of c-Jun N-terminal kinases through a phosphatidylinositol 3-kinase- and mitogen-activated protein kinase phosphatase-1-dependent pathway (2000) Endocrinology, 141, pp. 922-931; Dudhgaonkar, S., Thyagarajan, A., Sliva, D., Suppression of the inflammatory response by triterpenes isolated from the mushroom Ganoderma lucidum (2009) Int Immunopharmacol, 9, pp. 1272-1280; Dzau, V.J., Braun-Dullaeus, R.C., Sedding, D.G., Vascular proliferation and atherosclerosis: New perspectives and therapeutic strategies (2002) Nat Med, 8, pp. 1249-1256; Evans, S., Dizeyi, N., Abrahamsson, P.A., Persson, J., The effect of a novel botanical agent TBS-101 on invasive prostate cancer in animal models (2009) Anticancer Res, 29, pp. 3917-3924; Gao, Y., Gao, H., Chan, E., Tang, W., Xu, A., Yang, H., Huang, M., Zhou, S., Antitumor activity and underlying mechanisms of ganopoly, the refined polysaccharides extracted from Ganoderma lucidum, in mice (2005) Immunol Invest, 34, pp. 171-198; Ghosh, S.S., Gehr, T.W., Ghosh, S., Fakhry, I., Sica, D.A., Lyall, V., Schoolwerth, A.C., PPARgamma ligand attenuates PDGF-induced mesangial cell proliferation: Role of MAP kinase (2003) Kidney Int, 64, pp. 52-62; Golias, C.H., Charalabopoulos, A., Charalabopoulos, K., Cell proliferation and cell cycle control: A mini review (2004) Int J Clin Pract, 58, pp. 1134-1141; Heldin, C.H., Westermark, B., Mechanism of action and in vivo role of platelet-derived growth factor (1999) Physiol Rev, 79, pp. 1283-1316; Hsu, H.Y., Hua, K.F., Lin, C.C., Lin, C.H., Hsu, J., Wong, C.H., Extract of Reishi polysaccharides induces cytokine expression via TLR4-modulated protein kinase signaling pathways (2004) J Immunol, 173, pp. 5989-5999; Ishizawa, K., Izawa-Ishizawa, Y., Ohnishi, S., Motobayashi, Y., Kawazoe, K., Hamano, S., Tsuchiya, K., Tamaki, T., Quercetin glucuronide inhibits cell migration and proliferation by platelet-derived growth factor in vascular smooth muscle cells (2009) J Pharmacol Sci, 109, pp. 257-264; Jiang, J., Slivova, V., Harvey, K., Valachovicova, T., Sliva, D., Ganoderma lucidum suppresses growth of breast cancer cells through the inhibition of Akt/NF-kappaB signaling (2004) Nutr Cancer, 49, pp. 209-216; Jiang, J., Slivova, V., Sliva, D., Ganoderma lucidum inhibits proliferation of human breast cancer cells by down-regulation of estrogen receptor and NF-kappaB signaling (2006) Int J Oncol, 29, pp. 695-703; Kim, H.S., Cho, H.J., Park, S.J., Park, K.W., Chae, I.H., Oh, B.H., Park, Y.B., Lee, M.M., The essential role of p21 in radiation-induced cell cycle arrest of vascular smooth muscle cell (2004) J Mol Cell Cardiol, 37, pp. 871-880; Lin, S.J., Shyue, S.K., Hung, Y.Y., Chen, Y.H., Ku, H.H., Chen, J.W., Tam, K.B., Chen, Y.L., Superoxide dismutase inhibits the expression of vascular cell adhesion molecule-1 and intracellular cell adhesion molecule-1 induced by tumor necrosis factor-alpha in human endothelial cells through the JNK/p38 pathways (2005) Arterioscler Thromb Vasc Biol, 25, pp. 334-340; Lin, C.Y., Chen, Y.H., Hsu, H.Y., Wang, S.H., Liang, C.J., Kuan, I.I., Wu, P.J., Chen, Y.L., Ganoderma lucidum polysaccharides attenuate endotoxin-induced intercellular cell adhesion molecule-1 expression in cultured smooth muscle cells and in the neointima in mice (2010) J Agric Food Chem, 58, pp. 9563-9571; Miyazaki, T., Nishijima, M., Studies on fungal polysaccharides. 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year = "2012",
doi = "10.1002/jcp.23053",
language = "English",
volume = "227",
pages = "3063--3071",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "8",
}
