Gamma/delta T-cells enhance carboplatin-induced cytotoxicity towards advanced bladder cancer cells

Pan Yueh, Chiu Ya-Hsu, Chiu Shao-Chih, Cho Der-Yang, Lee Liang-Ming, Wen Yu-Ching, Whang Peng Jacqueline, Hsiao Chi-Hao, Shih Ping-Hsiao

Research output: Contribution to journalArticlepeer-review

Abstract

Background/Aim: Bladder cancer (BLCA, urothelial bladder cancer) is one of the most common malignancies with increasing incidence and mortality worldwide. Poor diagnosis and the limitation of treatment is still an unmet need in clinical practice. γδ T-Cells have been paid increasing attention because of their potent cytotoxicity against tumors. Herein, we investigated the cytolytic effect of γδ T-cells in combination with the chemotherapeutic drug, carboplatin, against BLCA cells. Materials and Methods: The standard protocol for the induction and expansion of peripheral blood mononuclear cell-derived γδ T-cells was a zoledronic acid/interleukin-2-based medium system for 2 weeks. The cytotoxicity of γδ T-cells with and without carboplatin against BLCA cells was examined. Results: After incubation, T-cell receptor-positive γδ T-cells showed a natural killer cell-like phenotypic characteristic and dose-dependently increased cytotoxicity against BLCA cells. Interestingly, we found that in advanced BLCA cells, which

Original languageEnglish
Pages (from-to)5221-5227
Number of pages7
JournalAnticancer Research
Volume40
Issue number9
DOIs
Publication statusPublished - Sep 2020

Keywords

  • Bladder cancer
  • Chemotherapy
  • Cytotoxicity
  • Gamma/delta T-cell
  • Immunocellular therapy

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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