Gamma-irradiation-prepared low molecular weight hyaluronic acid promotes skin wound healing

Yu Chih Huang, Kuen Yu Huang, Wei Zhen Lew, Kang Hsin Fan, Wei Jen Chang, Haw Ming Huang

Research output: Contribution to journalArticle

Abstract

In this study, we prepared low-molecular-weight hyaluronic acid (LMWHA) powder by γ -irradiation. The chemical and physical properties of γ -irradiated LMWHA and the in vitro cellular growth experiments with γ -irradiated LMWHA were analyzed. Then, hyaluronic acid exposed to 20 kGy of γ -irradiation was used to fabricate a carboxymethyl cellulose (CMC)/LMWHA fabric for wound dressing. Our results showed that γ -irradiated LMWHA demonstrated a significant alteration in carbon-oxygen double bonding and can be detected using nuclear magnetic resonance and ultraviolet (UV)-visible (Vis) spectra. The γ -irradiated LMWHA exhibited strain rate-dependent Newton/non-Newton fluid biphasic viscosity. The viability of L929 skin fibroblasts improved upon co-culture with γ -irradiated LMWHA. In the in vivo animal experiments, skin wounds covered with dressings prepared by γ -irradiation revealed acceleration of wound healing after two days of healing. The results suggest that γ -irradiated LMWHA could be a potential source for the promotion of skin wound healing.

Original languageEnglish
Article number1214
JournalPolymers
Volume11
Issue number7
DOIs
Publication statusPublished - Jul 1 2019

Fingerprint

Hyaluronic acid
Hyaluronic Acid
Skin
Molecular weight
Irradiation
Carboxymethylcellulose Sodium
Fibroblasts
Cell culture
Powders
Chemical properties
Strain rate
Cellulose
Animals
Carbon
Physical properties
Experiments
Nuclear magnetic resonance
Viscosity
Oxygen
Fluids

Keywords

  • Gamma ray
  • Hyaluronic acid
  • Low molecular weight
  • Membrane
  • Wound healing

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

Cite this

Gamma-irradiation-prepared low molecular weight hyaluronic acid promotes skin wound healing. / Huang, Yu Chih; Huang, Kuen Yu; Lew, Wei Zhen; Fan, Kang Hsin; Chang, Wei Jen; Huang, Haw Ming.

In: Polymers, Vol. 11, No. 7, 1214, 01.07.2019.

Research output: Contribution to journalArticle

Huang, Yu Chih ; Huang, Kuen Yu ; Lew, Wei Zhen ; Fan, Kang Hsin ; Chang, Wei Jen ; Huang, Haw Ming. / Gamma-irradiation-prepared low molecular weight hyaluronic acid promotes skin wound healing. In: Polymers. 2019 ; Vol. 11, No. 7.
@article{a72f4b0d123947049b576a8835450396,
title = "Gamma-irradiation-prepared low molecular weight hyaluronic acid promotes skin wound healing",
abstract = "In this study, we prepared low-molecular-weight hyaluronic acid (LMWHA) powder by γ -irradiation. The chemical and physical properties of γ -irradiated LMWHA and the in vitro cellular growth experiments with γ -irradiated LMWHA were analyzed. Then, hyaluronic acid exposed to 20 kGy of γ -irradiation was used to fabricate a carboxymethyl cellulose (CMC)/LMWHA fabric for wound dressing. Our results showed that γ -irradiated LMWHA demonstrated a significant alteration in carbon-oxygen double bonding and can be detected using nuclear magnetic resonance and ultraviolet (UV)-visible (Vis) spectra. The γ -irradiated LMWHA exhibited strain rate-dependent Newton/non-Newton fluid biphasic viscosity. The viability of L929 skin fibroblasts improved upon co-culture with γ -irradiated LMWHA. In the in vivo animal experiments, skin wounds covered with dressings prepared by γ -irradiation revealed acceleration of wound healing after two days of healing. The results suggest that γ -irradiated LMWHA could be a potential source for the promotion of skin wound healing.",
keywords = "Gamma ray, Hyaluronic acid, Low molecular weight, Membrane, Wound healing",
author = "Huang, {Yu Chih} and Huang, {Kuen Yu} and Lew, {Wei Zhen} and Fan, {Kang Hsin} and Chang, {Wei Jen} and Huang, {Haw Ming}",
year = "2019",
month = "7",
day = "1",
doi = "10.3390/polym11071214",
language = "English",
volume = "11",
journal = "Polymers",
issn = "2073-4360",
publisher = "MDPI AG",
number = "7",

}

TY - JOUR

T1 - Gamma-irradiation-prepared low molecular weight hyaluronic acid promotes skin wound healing

AU - Huang, Yu Chih

AU - Huang, Kuen Yu

AU - Lew, Wei Zhen

AU - Fan, Kang Hsin

AU - Chang, Wei Jen

AU - Huang, Haw Ming

PY - 2019/7/1

Y1 - 2019/7/1

N2 - In this study, we prepared low-molecular-weight hyaluronic acid (LMWHA) powder by γ -irradiation. The chemical and physical properties of γ -irradiated LMWHA and the in vitro cellular growth experiments with γ -irradiated LMWHA were analyzed. Then, hyaluronic acid exposed to 20 kGy of γ -irradiation was used to fabricate a carboxymethyl cellulose (CMC)/LMWHA fabric for wound dressing. Our results showed that γ -irradiated LMWHA demonstrated a significant alteration in carbon-oxygen double bonding and can be detected using nuclear magnetic resonance and ultraviolet (UV)-visible (Vis) spectra. The γ -irradiated LMWHA exhibited strain rate-dependent Newton/non-Newton fluid biphasic viscosity. The viability of L929 skin fibroblasts improved upon co-culture with γ -irradiated LMWHA. In the in vivo animal experiments, skin wounds covered with dressings prepared by γ -irradiation revealed acceleration of wound healing after two days of healing. The results suggest that γ -irradiated LMWHA could be a potential source for the promotion of skin wound healing.

AB - In this study, we prepared low-molecular-weight hyaluronic acid (LMWHA) powder by γ -irradiation. The chemical and physical properties of γ -irradiated LMWHA and the in vitro cellular growth experiments with γ -irradiated LMWHA were analyzed. Then, hyaluronic acid exposed to 20 kGy of γ -irradiation was used to fabricate a carboxymethyl cellulose (CMC)/LMWHA fabric for wound dressing. Our results showed that γ -irradiated LMWHA demonstrated a significant alteration in carbon-oxygen double bonding and can be detected using nuclear magnetic resonance and ultraviolet (UV)-visible (Vis) spectra. The γ -irradiated LMWHA exhibited strain rate-dependent Newton/non-Newton fluid biphasic viscosity. The viability of L929 skin fibroblasts improved upon co-culture with γ -irradiated LMWHA. In the in vivo animal experiments, skin wounds covered with dressings prepared by γ -irradiation revealed acceleration of wound healing after two days of healing. The results suggest that γ -irradiated LMWHA could be a potential source for the promotion of skin wound healing.

KW - Gamma ray

KW - Hyaluronic acid

KW - Low molecular weight

KW - Membrane

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=85070383262&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070383262&partnerID=8YFLogxK

U2 - 10.3390/polym11071214

DO - 10.3390/polym11071214

M3 - Article

AN - SCOPUS:85070383262

VL - 11

JO - Polymers

JF - Polymers

SN - 2073-4360

IS - 7

M1 - 1214

ER -