Galectin-3 level and the severity of cardiac diastolic dysfunction using cellular and animal models and clinical indices

Cho Kai Wu, Mao Yuan Su, Jen Kuang Lee, Fu Tien Chiang, Juey Jen Hwang, Jiunn Lee Lin, Jin Jer Chen, Fu Tong Liu, Chia Ti Tsai

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Abstract

Heart failure with preserved ejection fraction (HFPEF) is characterized by myocardial interstitial fibrosis. A total of 146 patients with HFPEF, were recruited. HFPEF severity was determined using Doppler imaging (E/Em) and also cardiac magnetic resonance imaging (CMRI). Canine modeling of HFPEF was induced by aortic banding. Hemodynamic and echocardiographic data were obtained before and after pressure loading and myocardial Galectin-3 was determined. Mechanical stretch of cultured cardiomyocytes served as the cellular model of HFPEF. Patients with severe HFPEF had significantly higher plasma Galectin-3 levels. Significant correlation between plasma Galectin-3 and E/Em in advanced HFPEF patients was noted. After 2 weeks of pressure overload in canine models, the protein expression of Galectin-3 from LV myocardial tissue was significantly increased (p < 0.01) compared with controls. Galectin-3 expression paralleled the severity of LV diastolic dysfunction by evaluation of CMRI (r =-0.58, p = 0.003) and tissue fibrosis (r = 0.59, p = 0.002). After adjusting for confounders for diastolic dysfunction, Galectin-3 levels were still associated with diastolic parameters both in humans (p < 0.001) and canine model (p = 0.041). Mechanical stretch increased Galectin-3 secretion in cultured cardiomyocytes. Both plasma and myocardial Galectin-3 levels correlated with severity of cardiac diastolic dysfunction.

Original languageEnglish
Article number17007
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - Nov 19 2015
Externally publishedYes

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Wu, C. K., Su, M. Y., Lee, J. K., Chiang, F. T., Hwang, J. J., Lin, J. L., Chen, J. J., Liu, F. T., & Tsai, C. T. (2015). Galectin-3 level and the severity of cardiac diastolic dysfunction using cellular and animal models and clinical indices. Scientific Reports, 5, [17007]. https://doi.org/10.1038/srep17007