Galectin-1, a novel ligand of neuropilin-1, activates VEGFR-2 signaling and modulates the migration of vascular endothelial cells

S. H. Hsieh, N. W. Ying, M. H. Wu, W. F. Chiang, C. L. Hsu, T. Y. Wong, Y. T. Jin, T. M. Hong, Y. L. Chen

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Galectin-1 (Gal-1), a homodimeric prototype of the galectins with a single carbohydrate-recognition domain, was recently identified as being overexpressed in tumor-associated capillary endothelial cells. The role of Gal-1 in endothelial cellular functions and the mechanism of action of Gal-1 remain unknown. Neuropilin-1 (NRP1) is a neuronal receptor that mediates repulsive growth cone guidance, and NRP1 functions in endothelial cells as a coreceptor (with vascular endothelial growth factor receptors (VEGFRs)) for VEGF 165. In this study, we found that Gal-1 was overexpressed in the tumor-associated endothelial cells of oral squamous cell carcinomas (P165. Surprisingly, Gal-1 selectively bound NRP1 via the carbohydrate-recognition domain, but did not bind VEGFR-1, VEGFR-2 or VEGFR-3. The Gal-1-NRP1 interaction mediated the migration and adhesion of endothelial cells. The binding of Gal-1 to NRP1 enhanced VEGFR-2 phosphorylation and stimulated the activation of the mitogen activated protein (MAP) kinases SAPK1/JNK (stress activated protein kinase-1/c-Jun NH2-terminal kinase). These findings show, for the first time, that Gal-1 can directly bind to NRP1 on endothelial cells, and can promote the NRP1/VEGFR-2-mediated signaling pathway as well as NRP1-mediated biological activities.

Original languageEnglish
Pages (from-to)3746-3753
Number of pages8
JournalOncogene
Volume27
Issue number26
DOIs
Publication statusPublished - Jun 12 2008
Externally publishedYes

Fingerprint

Neuropilin-1
Galectin 1
Vascular Endothelial Growth Factor Receptor-2
Endothelial Cells
Ligands
Carbohydrates
Vascular Endothelial Growth Factor Receptor-3
MAP Kinase Kinase 4
Galectins
Vascular Endothelial Growth Factor Receptor-1
Growth Cones
Vascular Endothelial Growth Factor Receptor
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases
Squamous Cell Carcinoma
Neoplasms
Phosphorylation

Keywords

  • Endothelial cells
  • Galectin-1
  • Neuropilin-1
  • Oral cancer

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Galectin-1, a novel ligand of neuropilin-1, activates VEGFR-2 signaling and modulates the migration of vascular endothelial cells. / Hsieh, S. H.; Ying, N. W.; Wu, M. H.; Chiang, W. F.; Hsu, C. L.; Wong, T. Y.; Jin, Y. T.; Hong, T. M.; Chen, Y. L.

In: Oncogene, Vol. 27, No. 26, 12.06.2008, p. 3746-3753.

Research output: Contribution to journalArticle

Hsieh, SH, Ying, NW, Wu, MH, Chiang, WF, Hsu, CL, Wong, TY, Jin, YT, Hong, TM & Chen, YL 2008, 'Galectin-1, a novel ligand of neuropilin-1, activates VEGFR-2 signaling and modulates the migration of vascular endothelial cells', Oncogene, vol. 27, no. 26, pp. 3746-3753. https://doi.org/10.1038/sj.onc.1211029
Hsieh, S. H. ; Ying, N. W. ; Wu, M. H. ; Chiang, W. F. ; Hsu, C. L. ; Wong, T. Y. ; Jin, Y. T. ; Hong, T. M. ; Chen, Y. L. / Galectin-1, a novel ligand of neuropilin-1, activates VEGFR-2 signaling and modulates the migration of vascular endothelial cells. In: Oncogene. 2008 ; Vol. 27, No. 26. pp. 3746-3753.
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