Gadolinium-based CuInS2/ZnS nanoprobe for dual-modality magnetic resonance/optical imaging

Chun Yi Cheng, Keng Liang Ou, Wei Ting Huang, Jem Kun Chen, Jia Yaw Chang, Cheng Hsien Yang

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A new magnetic resonance/optical nanoprobe with specific cellular targeting capabilities based on nontoxic CuInS2/ZnS quantum dots (QDs) with direct covalent attachment of a Gd(III)-complex for tumor-specific imaging is reported. We introduce amphiphilic poly(maleic anhydride-alt-1-octadecene) to interdigitate with hydrophobic, protective agents on the surface of CuInS 2/ZnS QDs that allows phase transfer of hydrophobic QDs from the organic into aqueous phase. Carbodiimide chemistry is used to covalently couple the Gd(III) complex on the surface of CuInS2/ZnS QDs, and then folic acid is further utilized to functionalize this dual-modality nanoprobe for active tumor targeting based on the fact that the membrane-associated folate receptor is overexpressed in many tumor cells. The longitudinal relaxivity value is 3.72 mM-1 s-1 for the dual-modality nanoprobe and a clear, positive, and increasing contrast enhancement of magnetic resonance signals concurrently with increasing Gd(III) concentration is observed. The dual-modality nanoprobe exhibits negligible cytotoxicity with >80% cell viability at a concentration of up to 100 μg/mL in human cervical (HeLa), human liver carcinoma (HepG2), and human breast (MCF-7) cells after 24 h. The specificity of folic-acid-conjugated nanoprobe cellular uptake has been investigated by confocal scanning laser imaging, which revealed that HeLa cells, expressing the folate receptor, internalized a higher level of dual-modality nanoprobes than HepG2 and MCF-7 cells.

Original languageEnglish
Pages (from-to)4389-4400
Number of pages12
JournalACS Applied Materials and Interfaces
Volume5
Issue number10
DOIs
Publication statusPublished - May 22 2013

Fingerprint

Nanoprobes
Gadolinium
Magnetic resonance
Imaging techniques
Folic Acid
Semiconductor quantum dots
Tumors
Cells
Maleic Anhydrides
Carbodiimides
Protective Agents
Acids
Maleic anhydride
Cytotoxicity
Liver
Membranes
Scanning
Lasers

Keywords

  • CuInS/ZnS
  • Gd(III)-complex
  • magnetic resonance imaging
  • optical imaging

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Cheng, C. Y., Ou, K. L., Huang, W. T., Chen, J. K., Chang, J. Y., & Yang, C. H. (2013). Gadolinium-based CuInS2/ZnS nanoprobe for dual-modality magnetic resonance/optical imaging. ACS Applied Materials and Interfaces, 5(10), 4389-4400. https://doi.org/10.1021/am401428n

Gadolinium-based CuInS2/ZnS nanoprobe for dual-modality magnetic resonance/optical imaging. / Cheng, Chun Yi; Ou, Keng Liang; Huang, Wei Ting; Chen, Jem Kun; Chang, Jia Yaw; Yang, Cheng Hsien.

In: ACS Applied Materials and Interfaces, Vol. 5, No. 10, 22.05.2013, p. 4389-4400.

Research output: Contribution to journalArticle

Cheng, Chun Yi ; Ou, Keng Liang ; Huang, Wei Ting ; Chen, Jem Kun ; Chang, Jia Yaw ; Yang, Cheng Hsien. / Gadolinium-based CuInS2/ZnS nanoprobe for dual-modality magnetic resonance/optical imaging. In: ACS Applied Materials and Interfaces. 2013 ; Vol. 5, No. 10. pp. 4389-4400.
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