GABA tea attenuates cardiac apoptosis in spontaneously hypertensive rats (SHR) by enhancing PI3K/Akt-mediated survival pathway and suppressing Bax/Bak dependent apoptotic pathway

Bih Cheng Chen, Meng Yu Hung, Hsueh Fang Wang, Li Jen Yeh, Sudhir Pandey, Ray Jade Chen, Ruey Lin Chang, Vijaya Padma Viswanadha, Kuan Ho Lin, Chih Yang Huang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cardiomyocyte apoptosis is the major risk factor for the development of heart failure (HF). The purpose of this study was to evaluate the effects of Gamma-aminobutyric acid (GABA) tea on hypertension-induced cardiac apoptotic pathways in spontaneously hypertensive rats (SHR). In order to reveal the mechanisms, 36 male SHR at eight weeks of age, 200 g were divided into six groups. One group was fed water as a control group. Other rats were administered one of the following treatments: GABA tea at dose 150 and 300 mg/kg/day as low GABA tea (LGT) and high GABA tea (HGT) groups, respectively, pure GABA at dose 150 and 300 mg/kg/day as LG and HG groups, respectively, green tea (GT) as control of LGT and HGT groups. After 12 weeks, cardiac tissues were analyzed by histological analysis, western blotting, and TUNEL assays. GABA tea, GT, and pure GABA decreased hypertension-induced cardiac abnormalities, including abnormal myocardial architecture. In addition, GABA tea, GT, and pure GABA dramatically increased anti-apoptotic protein, Bcl2. Furthermore, GABA tea, GT, and pure GABA also decreased activated-caspase 9 and activated-caspase 3. Additionally, the survival associated protein IGF-I and PI3K/Akt were enhanced in cardiac tissues upon treatment. Our results showed an optimistic anti-apoptotic and pro-survival effects of GABA tea treatment against hypertensive rat hearts.

Original languageEnglish
JournalEnvironmental Toxicology
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

apoptosis
tea
Tea
Inbred SHR Rats
Phosphatidylinositol 3-Kinases
gamma-Aminobutyric Acid
Rats
Apoptosis
acid
hypertension
Tissue
Hypertension
Apoptosis Regulatory Proteins
protein
Caspase 9
In Situ Nick-End Labeling
abnormality
Insulin-Like Growth Factor I
risk factor
Cardiac Myocytes

Keywords

  • Apoptosis
  • Bak
  • Bax
  • BCL2
  • Cardiac hypertension
  • GABA tea
  • P-Akt

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

Cite this

GABA tea attenuates cardiac apoptosis in spontaneously hypertensive rats (SHR) by enhancing PI3K/Akt-mediated survival pathway and suppressing Bax/Bak dependent apoptotic pathway. / Chen, Bih Cheng; Hung, Meng Yu; Wang, Hsueh Fang; Yeh, Li Jen; Pandey, Sudhir; Chen, Ray Jade; Chang, Ruey Lin; Viswanadha, Vijaya Padma; Lin, Kuan Ho; Huang, Chih Yang.

In: Environmental Toxicology, 01.01.2018.

Research output: Contribution to journalArticle

Chen, Bih Cheng ; Hung, Meng Yu ; Wang, Hsueh Fang ; Yeh, Li Jen ; Pandey, Sudhir ; Chen, Ray Jade ; Chang, Ruey Lin ; Viswanadha, Vijaya Padma ; Lin, Kuan Ho ; Huang, Chih Yang. / GABA tea attenuates cardiac apoptosis in spontaneously hypertensive rats (SHR) by enhancing PI3K/Akt-mediated survival pathway and suppressing Bax/Bak dependent apoptotic pathway. In: Environmental Toxicology. 2018.
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abstract = "Cardiomyocyte apoptosis is the major risk factor for the development of heart failure (HF). The purpose of this study was to evaluate the effects of Gamma-aminobutyric acid (GABA) tea on hypertension-induced cardiac apoptotic pathways in spontaneously hypertensive rats (SHR). In order to reveal the mechanisms, 36 male SHR at eight weeks of age, 200 g were divided into six groups. One group was fed water as a control group. Other rats were administered one of the following treatments: GABA tea at dose 150 and 300 mg/kg/day as low GABA tea (LGT) and high GABA tea (HGT) groups, respectively, pure GABA at dose 150 and 300 mg/kg/day as LG and HG groups, respectively, green tea (GT) as control of LGT and HGT groups. After 12 weeks, cardiac tissues were analyzed by histological analysis, western blotting, and TUNEL assays. GABA tea, GT, and pure GABA decreased hypertension-induced cardiac abnormalities, including abnormal myocardial architecture. In addition, GABA tea, GT, and pure GABA dramatically increased anti-apoptotic protein, Bcl2. Furthermore, GABA tea, GT, and pure GABA also decreased activated-caspase 9 and activated-caspase 3. Additionally, the survival associated protein IGF-I and PI3K/Akt were enhanced in cardiac tissues upon treatment. Our results showed an optimistic anti-apoptotic and pro-survival effects of GABA tea treatment against hypertensive rat hearts.",
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AU - Wang, Hsueh Fang

AU - Yeh, Li Jen

AU - Pandey, Sudhir

AU - Chen, Ray Jade

AU - Chang, Ruey Lin

AU - Viswanadha, Vijaya Padma

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AU - Huang, Chih Yang

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