Functional study of hepatitis delta virus large antigen in packaging and replication inhibition

Role of the amino-terminal leucine zipper

Pei Jer Chen, Fu Lin Chang, Chuan Jen Wang, Chien Ju Lin, Shian Ying Sung, Ding Shinn Chen

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The large hepatitis delta antigen (HDAg) has been found to be essential for the assembly of the hepatitis delta virion. Furthermore, in a cotransfection experiment, the large HDAg itself, without the hepatitis delta virus (HDV) genome and small HDAg, could be packaged into hepatitis B surface antigen (HBsAg) particles. By deletion analysis, it was shown that the amino-terminal leucine zipper domain was dispensable for packaging. The large HDAg could also help in copackaging of the small HDAg into HBsAg particles without the need for HDV RNA. This process was probably mediated through direct interaction of the two HDAgs as a mutated large HDAg whose leucine zipper domain was deleted such that it could not help in copackaging of the small HDAg. This mutated large HDAg did not suppress HDV replication, suggesting that this effect is probably also via protein interaction. These results indicated that functional domains of the large HDAg responsible for packaging with HBsAg particles and for the trans-negative effect on HDV replication can be separated.

Original languageEnglish
Pages (from-to)2853-2859
Number of pages7
JournalJournal of Virology
Volume66
Issue number5
Publication statusPublished - May 1992
Externally publishedYes

Fingerprint

Hepatitis delta virus
Leucine Zippers
leucine zipper
hepatitis
Product Packaging
Hepatitis delta Antigens
packaging
Hepatitis Delta Virus
antigens
Hepatitis B Surface Antigens
Virus Replication
Hepatitis D
virus replication
Virion
hepatitis delta virus large antigen
Genome
RNA
virion

ASJC Scopus subject areas

  • Immunology

Cite this

Functional study of hepatitis delta virus large antigen in packaging and replication inhibition : Role of the amino-terminal leucine zipper. / Chen, Pei Jer; Chang, Fu Lin; Wang, Chuan Jen; Lin, Chien Ju; Sung, Shian Ying; Chen, Ding Shinn.

In: Journal of Virology, Vol. 66, No. 5, 05.1992, p. 2853-2859.

Research output: Contribution to journalArticle

Chen, Pei Jer ; Chang, Fu Lin ; Wang, Chuan Jen ; Lin, Chien Ju ; Sung, Shian Ying ; Chen, Ding Shinn. / Functional study of hepatitis delta virus large antigen in packaging and replication inhibition : Role of the amino-terminal leucine zipper. In: Journal of Virology. 1992 ; Vol. 66, No. 5. pp. 2853-2859.
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