Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment

Jiun Lin Horng, Li Yih Lin, Pung Pung Hwang

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

It is important to maintain internal pH homeostasis in biological systems. In our previous studies, H+-ATPase-rich (HR) cells were found to be responsible for proton secretion in the skin of zebrafish embryos during development. In this study, zebrafish embryos were exposed to acidic and basic waters to investigate the regulation of HR cell acid secretion during pH disturbances. Our results showed that the function of HR cells on the skin of zebrafish embryos can be upregulated in pH 4 water not only by increasing the cell number but also by enlarging the acid-secreting function of single cells. We also identified an "alveolar-type" apical opening under scanning electron microscopy observations of the apical membrane of HR cells, and the density and size of the alveolar type of apical openings were also increased in pH 4 water. p63 and PCNA immunostaining results also showed that additional HR cells in pH 4 water may be differentiated not only from ionocyte precursor cells but also newly proliferating epithelial stem cells.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume296
Issue number4
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

Fingerprint

Proton-Translocating ATPases
Zebrafish
Embryonic Structures
Water
Cell Count
Skin
Acids
Proliferating Cell Nuclear Antigen
Cell Size
Electron Scanning Microscopy
Embryonic Development
Protons
Homeostasis
Stem Cells
Epithelial Cells
Membranes

Keywords

  • Acid-base regulation
  • Differentiation
  • Ion regulation
  • Ionocytes
  • Proliferation

ASJC Scopus subject areas

  • Cell Biology
  • Physiology

Cite this

Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment. / Horng, Jiun Lin; Lin, Li Yih; Hwang, Pung Pung.

In: American Journal of Physiology - Cell Physiology, Vol. 296, No. 4, 04.2009.

Research output: Contribution to journalArticle

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