Functional domains of pseudomonas exotoxin identified by deletion analysis of the gene expressed in E. coli

Jaulang Hwang, David J. Fitzgerald, Sankar Adhya, Ira Pastan

Research output: Contribution to journalArticle

332 Citations (Scopus)

Abstract

Pseudomonas exotoxin A is a single chain toxin with three structural domains that inhibits protein synthesis in eukaryotic cells by catalyzing ADP ribosylation of elongation factor 2. To study the function of these domains, we deleted different portions of the PE structural gene and expressed these constructs in E. coli using an inducible T7 promoter. These studies indicate that structural domain la is required for cell recognition, that structural domain ll is required to translocate the toxin across a cellular membrane, and that structural domain III and a portion of domain lb are required for ADP ribosylation activity. Toxin lacking domain la is about 100-fold less toxic to mice than intact PE and should be a useful molecule for the construction of immunotoxins.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalCell
Volume48
Issue number1
DOIs
Publication statusPublished - Jan 16 1987
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Functional domains of pseudomonas exotoxin identified by deletion analysis of the gene expressed in E. coli'. Together they form a unique fingerprint.

  • Cite this