FTY720 mitigates torsion/detorsion-induced testicular injury in rats

Hung Jen Shih, Jiin Cherng Yen, Allen W. Chiu, Yung Chiong Chow, Wynn H.T. Pan, Tao Yeuan Wang, Chun Jen Huang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background FTY720, a sphingosine-1-phosphate (S1P) receptor agonist, possesses potent anti-inflammation capacity. We evaluated the therapeutic potentials of FTY720 against testicular injury induced by testicular torsion and/or detorsion (T/D). Materials and methods Young adult male Sprague-Dawley rats were allocated to receive T/D (the T/D group) and T/D plus FTY720 (4 mg/kg, the T/D-FTY group, n = 6 in each group). To investigate the possible roles of the S1P receptors, another group of rats received T/D plus FTY720 plus the potent S1P receptor antagonist VPC23019 (1 mg/kg, the T/D-FTY-VPC group, n = 6). FTY720 was administered immediately before testicular detorsion, and VPC23019 was administered 30 min before FTY720. Another set of rats that received sham operation, immediately followed by injection of normal saline, FTY720, or FTY720 plus VPC23019, served as control groups. Sham control groups were run simultaneously. After euthanization, levels of testicular injury were measured. Results Histologic findings revealed severe testicular injury changes in both the T/D and T/D-FTY-VPC groups and moderate testicular injury changes in the T/D-FTY group. In addition, malondialdehyde activity (oxidative status), concentration of interleukin-1β (inflammation index), myeloperoxidase activity (neutrophil infiltration index), and wet-to-dry weight ratio (tissue edema index) of both the T/D and T/D-FTY-VPC groups were significantly higher than those of the T/D-FTY group. These data confirmed the protective effects of FTY720 against testicular T/D. Moreover, antagonizing the S1P receptors could reverse the protective effects of FTY720. Conclusions FTY720 significantly mitigated testicular injury induced by testicular T/D. The mechanisms may involve activating the S1P receptors.

Original languageEnglish
Pages (from-to)328-331
Number of pages4
JournalJournal of Surgical Research
Volume196
Issue number2
DOIs
Publication statusPublished - Jun 15 2015
Externally publishedYes

Fingerprint

Lysosphingolipid Receptors
Wounds and Injuries
Fingolimod Hydrochloride
Spermatic Cord Torsion
Inflammation
Control Groups
Neutrophil Infiltration
Malondialdehyde
Interleukin-1
Peroxidase
Sprague Dawley Rats
Young Adult
Edema
Weights and Measures
Injections
VPC23019

Keywords

  • Ischemia
  • Reperfusion
  • Sphingosine-1-phosphate
  • Testis
  • Torsion

ASJC Scopus subject areas

  • Surgery

Cite this

FTY720 mitigates torsion/detorsion-induced testicular injury in rats. / Shih, Hung Jen; Yen, Jiin Cherng; Chiu, Allen W.; Chow, Yung Chiong; Pan, Wynn H.T.; Wang, Tao Yeuan; Huang, Chun Jen.

In: Journal of Surgical Research, Vol. 196, No. 2, 15.06.2015, p. 328-331.

Research output: Contribution to journalArticle

Shih, Hung Jen ; Yen, Jiin Cherng ; Chiu, Allen W. ; Chow, Yung Chiong ; Pan, Wynn H.T. ; Wang, Tao Yeuan ; Huang, Chun Jen. / FTY720 mitigates torsion/detorsion-induced testicular injury in rats. In: Journal of Surgical Research. 2015 ; Vol. 196, No. 2. pp. 328-331.
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abstract = "Background FTY720, a sphingosine-1-phosphate (S1P) receptor agonist, possesses potent anti-inflammation capacity. We evaluated the therapeutic potentials of FTY720 against testicular injury induced by testicular torsion and/or detorsion (T/D). Materials and methods Young adult male Sprague-Dawley rats were allocated to receive T/D (the T/D group) and T/D plus FTY720 (4 mg/kg, the T/D-FTY group, n = 6 in each group). To investigate the possible roles of the S1P receptors, another group of rats received T/D plus FTY720 plus the potent S1P receptor antagonist VPC23019 (1 mg/kg, the T/D-FTY-VPC group, n = 6). FTY720 was administered immediately before testicular detorsion, and VPC23019 was administered 30 min before FTY720. Another set of rats that received sham operation, immediately followed by injection of normal saline, FTY720, or FTY720 plus VPC23019, served as control groups. Sham control groups were run simultaneously. After euthanization, levels of testicular injury were measured. Results Histologic findings revealed severe testicular injury changes in both the T/D and T/D-FTY-VPC groups and moderate testicular injury changes in the T/D-FTY group. In addition, malondialdehyde activity (oxidative status), concentration of interleukin-1β (inflammation index), myeloperoxidase activity (neutrophil infiltration index), and wet-to-dry weight ratio (tissue edema index) of both the T/D and T/D-FTY-VPC groups were significantly higher than those of the T/D-FTY group. These data confirmed the protective effects of FTY720 against testicular T/D. Moreover, antagonizing the S1P receptors could reverse the protective effects of FTY720. Conclusions FTY720 significantly mitigated testicular injury induced by testicular T/D. The mechanisms may involve activating the S1P receptors.",
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author = "Shih, {Hung Jen} and Yen, {Jiin Cherng} and Chiu, {Allen W.} and Chow, {Yung Chiong} and Pan, {Wynn H.T.} and Wang, {Tao Yeuan} and Huang, {Chun Jen}",
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T1 - FTY720 mitigates torsion/detorsion-induced testicular injury in rats

AU - Shih, Hung Jen

AU - Yen, Jiin Cherng

AU - Chiu, Allen W.

AU - Chow, Yung Chiong

AU - Pan, Wynn H.T.

AU - Wang, Tao Yeuan

AU - Huang, Chun Jen

PY - 2015/6/15

Y1 - 2015/6/15

N2 - Background FTY720, a sphingosine-1-phosphate (S1P) receptor agonist, possesses potent anti-inflammation capacity. We evaluated the therapeutic potentials of FTY720 against testicular injury induced by testicular torsion and/or detorsion (T/D). Materials and methods Young adult male Sprague-Dawley rats were allocated to receive T/D (the T/D group) and T/D plus FTY720 (4 mg/kg, the T/D-FTY group, n = 6 in each group). To investigate the possible roles of the S1P receptors, another group of rats received T/D plus FTY720 plus the potent S1P receptor antagonist VPC23019 (1 mg/kg, the T/D-FTY-VPC group, n = 6). FTY720 was administered immediately before testicular detorsion, and VPC23019 was administered 30 min before FTY720. Another set of rats that received sham operation, immediately followed by injection of normal saline, FTY720, or FTY720 plus VPC23019, served as control groups. Sham control groups were run simultaneously. After euthanization, levels of testicular injury were measured. Results Histologic findings revealed severe testicular injury changes in both the T/D and T/D-FTY-VPC groups and moderate testicular injury changes in the T/D-FTY group. In addition, malondialdehyde activity (oxidative status), concentration of interleukin-1β (inflammation index), myeloperoxidase activity (neutrophil infiltration index), and wet-to-dry weight ratio (tissue edema index) of both the T/D and T/D-FTY-VPC groups were significantly higher than those of the T/D-FTY group. These data confirmed the protective effects of FTY720 against testicular T/D. Moreover, antagonizing the S1P receptors could reverse the protective effects of FTY720. Conclusions FTY720 significantly mitigated testicular injury induced by testicular T/D. The mechanisms may involve activating the S1P receptors.

AB - Background FTY720, a sphingosine-1-phosphate (S1P) receptor agonist, possesses potent anti-inflammation capacity. We evaluated the therapeutic potentials of FTY720 against testicular injury induced by testicular torsion and/or detorsion (T/D). Materials and methods Young adult male Sprague-Dawley rats were allocated to receive T/D (the T/D group) and T/D plus FTY720 (4 mg/kg, the T/D-FTY group, n = 6 in each group). To investigate the possible roles of the S1P receptors, another group of rats received T/D plus FTY720 plus the potent S1P receptor antagonist VPC23019 (1 mg/kg, the T/D-FTY-VPC group, n = 6). FTY720 was administered immediately before testicular detorsion, and VPC23019 was administered 30 min before FTY720. Another set of rats that received sham operation, immediately followed by injection of normal saline, FTY720, or FTY720 plus VPC23019, served as control groups. Sham control groups were run simultaneously. After euthanization, levels of testicular injury were measured. Results Histologic findings revealed severe testicular injury changes in both the T/D and T/D-FTY-VPC groups and moderate testicular injury changes in the T/D-FTY group. In addition, malondialdehyde activity (oxidative status), concentration of interleukin-1β (inflammation index), myeloperoxidase activity (neutrophil infiltration index), and wet-to-dry weight ratio (tissue edema index) of both the T/D and T/D-FTY-VPC groups were significantly higher than those of the T/D-FTY group. These data confirmed the protective effects of FTY720 against testicular T/D. Moreover, antagonizing the S1P receptors could reverse the protective effects of FTY720. Conclusions FTY720 significantly mitigated testicular injury induced by testicular T/D. The mechanisms may involve activating the S1P receptors.

KW - Ischemia

KW - Reperfusion

KW - Sphingosine-1-phosphate

KW - Testis

KW - Torsion

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