Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats

Shi Jye Chu, Deh Ming Chang, David Wang, Ying Hsin Chen, Chin Wang Hsu, Kang Hsu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Objective: To determine whether fructose-1,6-diphosphate (FDP) pretreatment can attenuate acute lung injury induced by ischemia-reperfusion in our isolated lung model in rats. Design: Randomized, controlled study. Setting: Animal care facility procedure room. Subjects: Twenty-four adult male Sprague-Dawley rats each weighing 250-350 g. Interventions: Typical acute lung injury in rats was induced successfully by 10 mins of hypoxia followed by 75 mins of ischemia and 50 mins of reperfusion. Ischemia-reperfusion significantly increased microvascular permeability as measured by the capillary filtration coefficient, lung weight gain, lung weight to body weight ratio, pulmonary arterial pressure, and protein concentration of bronchoalveolar lavage fluid. Measurements and Main Results: Pretreatment with FDP significantly attenuated the acute lung injury induced by ischemia-reperfusion as shown by a significant decrease in all of the assessed variables (p <.05 ∼ p - .001). The protective effect of FDP was nearly undetectable when promazine (an ecto-adenosine 5′-triphosphatase inhibitor) was added before FDP pretreatment. Conclusions: Pretreatment with FDP significantly ameliorates acute lung injury induced by ischemia-reperfusion in rats.

Original languageEnglish
Pages (from-to)1605-1609
Number of pages5
JournalCritical Care Medicine
Volume30
Issue number7
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Acute Lung Injury
Reperfusion
Ischemia
Lung
Promazine
Bronchoalveolar Lavage Fluid
Capillary Permeability
Weight Gain
Sprague Dawley Rats
Arterial Pressure
Body Weight
fructose-1,6-diphosphate
Weights and Measures
Proteins

Keywords

  • Acute lung injury
  • ecto-ATPase
  • Fructose-1,6-diphosphate
  • Ischemia-reperfusion
  • Oxygen radicals
  • Promazine

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Chu, S. J., Chang, D. M., Wang, D., Chen, Y. H., Hsu, C. W., & Hsu, K. (2002). Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats. Critical Care Medicine, 30(7), 1605-1609.

Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats. / Chu, Shi Jye; Chang, Deh Ming; Wang, David; Chen, Ying Hsin; Hsu, Chin Wang; Hsu, Kang.

In: Critical Care Medicine, Vol. 30, No. 7, 2002, p. 1605-1609.

Research output: Contribution to journalArticle

Chu, SJ, Chang, DM, Wang, D, Chen, YH, Hsu, CW & Hsu, K 2002, 'Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats', Critical Care Medicine, vol. 30, no. 7, pp. 1605-1609.
Chu, Shi Jye ; Chang, Deh Ming ; Wang, David ; Chen, Ying Hsin ; Hsu, Chin Wang ; Hsu, Kang. / Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats. In: Critical Care Medicine. 2002 ; Vol. 30, No. 7. pp. 1605-1609.
@article{d65487b229544ce9906898bd64bda937,
title = "Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats",
abstract = "Objective: To determine whether fructose-1,6-diphosphate (FDP) pretreatment can attenuate acute lung injury induced by ischemia-reperfusion in our isolated lung model in rats. Design: Randomized, controlled study. Setting: Animal care facility procedure room. Subjects: Twenty-four adult male Sprague-Dawley rats each weighing 250-350 g. Interventions: Typical acute lung injury in rats was induced successfully by 10 mins of hypoxia followed by 75 mins of ischemia and 50 mins of reperfusion. Ischemia-reperfusion significantly increased microvascular permeability as measured by the capillary filtration coefficient, lung weight gain, lung weight to body weight ratio, pulmonary arterial pressure, and protein concentration of bronchoalveolar lavage fluid. Measurements and Main Results: Pretreatment with FDP significantly attenuated the acute lung injury induced by ischemia-reperfusion as shown by a significant decrease in all of the assessed variables (p <.05 ∼ p - .001). The protective effect of FDP was nearly undetectable when promazine (an ecto-adenosine 5′-triphosphatase inhibitor) was added before FDP pretreatment. Conclusions: Pretreatment with FDP significantly ameliorates acute lung injury induced by ischemia-reperfusion in rats.",
keywords = "Acute lung injury, ecto-ATPase, Fructose-1,6-diphosphate, Ischemia-reperfusion, Oxygen radicals, Promazine",
author = "Chu, {Shi Jye} and Chang, {Deh Ming} and David Wang and Chen, {Ying Hsin} and Hsu, {Chin Wang} and Kang Hsu",
year = "2002",
language = "English",
volume = "30",
pages = "1605--1609",
journal = "Critical Care Medicine",
issn = "0090-3493",
publisher = "Lippincott Williams and Wilkins",
number = "7",

}

TY - JOUR

T1 - Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats

AU - Chu, Shi Jye

AU - Chang, Deh Ming

AU - Wang, David

AU - Chen, Ying Hsin

AU - Hsu, Chin Wang

AU - Hsu, Kang

PY - 2002

Y1 - 2002

N2 - Objective: To determine whether fructose-1,6-diphosphate (FDP) pretreatment can attenuate acute lung injury induced by ischemia-reperfusion in our isolated lung model in rats. Design: Randomized, controlled study. Setting: Animal care facility procedure room. Subjects: Twenty-four adult male Sprague-Dawley rats each weighing 250-350 g. Interventions: Typical acute lung injury in rats was induced successfully by 10 mins of hypoxia followed by 75 mins of ischemia and 50 mins of reperfusion. Ischemia-reperfusion significantly increased microvascular permeability as measured by the capillary filtration coefficient, lung weight gain, lung weight to body weight ratio, pulmonary arterial pressure, and protein concentration of bronchoalveolar lavage fluid. Measurements and Main Results: Pretreatment with FDP significantly attenuated the acute lung injury induced by ischemia-reperfusion as shown by a significant decrease in all of the assessed variables (p <.05 ∼ p - .001). The protective effect of FDP was nearly undetectable when promazine (an ecto-adenosine 5′-triphosphatase inhibitor) was added before FDP pretreatment. Conclusions: Pretreatment with FDP significantly ameliorates acute lung injury induced by ischemia-reperfusion in rats.

AB - Objective: To determine whether fructose-1,6-diphosphate (FDP) pretreatment can attenuate acute lung injury induced by ischemia-reperfusion in our isolated lung model in rats. Design: Randomized, controlled study. Setting: Animal care facility procedure room. Subjects: Twenty-four adult male Sprague-Dawley rats each weighing 250-350 g. Interventions: Typical acute lung injury in rats was induced successfully by 10 mins of hypoxia followed by 75 mins of ischemia and 50 mins of reperfusion. Ischemia-reperfusion significantly increased microvascular permeability as measured by the capillary filtration coefficient, lung weight gain, lung weight to body weight ratio, pulmonary arterial pressure, and protein concentration of bronchoalveolar lavage fluid. Measurements and Main Results: Pretreatment with FDP significantly attenuated the acute lung injury induced by ischemia-reperfusion as shown by a significant decrease in all of the assessed variables (p <.05 ∼ p - .001). The protective effect of FDP was nearly undetectable when promazine (an ecto-adenosine 5′-triphosphatase inhibitor) was added before FDP pretreatment. Conclusions: Pretreatment with FDP significantly ameliorates acute lung injury induced by ischemia-reperfusion in rats.

KW - Acute lung injury

KW - ecto-ATPase

KW - Fructose-1,6-diphosphate

KW - Ischemia-reperfusion

KW - Oxygen radicals

KW - Promazine

UR - http://www.scopus.com/inward/record.url?scp=0036314084&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036314084&partnerID=8YFLogxK

M3 - Article

VL - 30

SP - 1605

EP - 1609

JO - Critical Care Medicine

JF - Critical Care Medicine

SN - 0090-3493

IS - 7

ER -