Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats

Shi Jye Chu, Deh Ming Chang, David Wang, Ying Hsin Chen, Chin Wang Hsu, Kang Hsu

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26 Citations (Scopus)

Abstract

Objective: To determine whether fructose-1,6-diphosphate (FDP) pretreatment can attenuate acute lung injury induced by ischemia-reperfusion in our isolated lung model in rats. Design: Randomized, controlled study. Setting: Animal care facility procedure room. Subjects: Twenty-four adult male Sprague-Dawley rats each weighing 250-350 g. Interventions: Typical acute lung injury in rats was induced successfully by 10 mins of hypoxia followed by 75 mins of ischemia and 50 mins of reperfusion. Ischemia-reperfusion significantly increased microvascular permeability as measured by the capillary filtration coefficient, lung weight gain, lung weight to body weight ratio, pulmonary arterial pressure, and protein concentration of bronchoalveolar lavage fluid. Measurements and Main Results: Pretreatment with FDP significantly attenuated the acute lung injury induced by ischemia-reperfusion as shown by a significant decrease in all of the assessed variables (p <.05 ∼ p - .001). The protective effect of FDP was nearly undetectable when promazine (an ecto-adenosine 5′-triphosphatase inhibitor) was added before FDP pretreatment. Conclusions: Pretreatment with FDP significantly ameliorates acute lung injury induced by ischemia-reperfusion in rats.

Original languageEnglish
Pages (from-to)1605-1609
Number of pages5
JournalCritical Care Medicine
Volume30
Issue number7
Publication statusPublished - 2002
Externally publishedYes

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Keywords

  • Acute lung injury
  • ecto-ATPase
  • Fructose-1,6-diphosphate
  • Ischemia-reperfusion
  • Oxygen radicals
  • Promazine

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Chu, S. J., Chang, D. M., Wang, D., Chen, Y. H., Hsu, C. W., & Hsu, K. (2002). Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats. Critical Care Medicine, 30(7), 1605-1609.