Free radical-scavenging phytochemicals of hot water extracts of Acacia confusa leaves detected by an on-line screening method

Yu Tang Tung, Jyh Horng Wu, Ching Yu Hsieh, Ping Sheng Chen, Shang Tzen Chang

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Acacia confusa Merr. (Leguminosae), a species native to Taiwan, is widely distributed on the hills and lowlands of Taiwan, and has been traditionally used as a medicine. In this study, phytochemicals and antioxidant activities of hot water extracts from A. confusa leaves were investigated for the first time. Among all the fractions from hot water extracts of leaves, the EtOAc-soluble fraction exhibits the best DPPH radical-scavenging activity, superoxide radical-scavenging activity, and reducing power. In addition, a rapid screening method, on-line RP-HPLC-DPPH system, for individual antioxidants in the EtOAc-soluble fraction was developed. Furthermore, following solid phase extraction (SPE) and reverse-phase high-performance liquid chromatography 12 pure phenolic compounds, including five major compounds (gallic acid, (+)-catechin, (-)-epicatechin, myricetin 3-glucopyranoside, and myricetin 3-rhamnopyranoside) were detected using the developed screening method. These results demonstrated that hot water extracts of A. confusa leaves have excellent antioxidant activities and thus have great potential as a source for natural health products.

Original languageEnglish
Pages (from-to)1019-1024
Number of pages6
JournalFood Chemistry
Volume115
Issue number3
DOIs
Publication statusPublished - Aug 1 2009
Externally publishedYes

Fingerprint

Acacia confusa
Acacia
Scavenging
Phytochemicals
phytopharmaceuticals
Free Radicals
Screening
myricetin
Catechin
Antioxidants
screening
Taiwan
Water
extracts
high performance liquid chromatography
antioxidant activity
High Pressure Liquid Chromatography
leaves
Gallic Acid
reversed-phase liquid chromatography

Keywords

  • Acacia confusa
  • Hot water extracts
  • Leaf
  • On-line RP-HPLC-DPPH
  • Phenolic compounds

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Medicine(all)

Cite this

Free radical-scavenging phytochemicals of hot water extracts of Acacia confusa leaves detected by an on-line screening method. / Tung, Yu Tang; Wu, Jyh Horng; Hsieh, Ching Yu; Chen, Ping Sheng; Chang, Shang Tzen.

In: Food Chemistry, Vol. 115, No. 3, 01.08.2009, p. 1019-1024.

Research output: Contribution to journalArticle

Tung, Yu Tang ; Wu, Jyh Horng ; Hsieh, Ching Yu ; Chen, Ping Sheng ; Chang, Shang Tzen. / Free radical-scavenging phytochemicals of hot water extracts of Acacia confusa leaves detected by an on-line screening method. In: Food Chemistry. 2009 ; Vol. 115, No. 3. pp. 1019-1024.
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