Abstract

Non-small cell lung cancer (NSCLC) is among the leading causes of human mortality. One reason for high rates of NSCLC mortality is that drug resistance is a major problem for both conventional chemotherapies and less-toxic targeted therapies. Thus, novel mechanistic insights into disease pathogenesis may benefit the development of urgently needed therapies. Here we show that FBJ murine osteosarcoma viral oncogene homolog B (FOSB) was induced by an antimicrobial peptide, tilapia piscidin-4 (TP4), through the dysregulation of mitochondrial Ca 2+ homeostasis in NSCLC cells. Transcriptomic, chromatin immunoprecipitation quantitative PCR, and immunocytochemical studies reveal that protocadherin-β13 (PCDHB13) as a target of FOSB that was functionally associated with microtubule. Overexpression of either PCDHB13 or FOSB attenuated NSCLC growth and survival in vitro and in vivo. Importantly, downregulation of both FOSB and PCDHB13 was observed in NSCLC patients and was negatively correlated with pathological grade. These findings introduce the FOSB-PCDHB13 axis as a novel tumor suppressive pathway in NSCLC.

Original languageEnglish
Article number107
JournalCancers
Volume11
Issue number1
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Non-Small Cell Lung Carcinoma
Microtubules
Tilapia
Mortality
Chromatin Immunoprecipitation
Poisons
Osteosarcoma
Oncogenes
Drug Resistance
Homeostasis
Down-Regulation
Drug Therapy
Polymerase Chain Reaction
Peptides
Survival
Therapeutics
Growth
Neoplasms

Keywords

  • antimicrobial peptide
  • Cytoskeleton
  • FOSB
  • Mitochondrial stress
  • Non-small cell lung cancer (NSCLC)
  • Protocadherin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

FOSB-PCDHB13 axis disrupts the microtubule network in non-small cell lung cancer. / Ting, Chen Hung; Lee, Kang Yun; Wu, Sheng Ming; Feng, Po Hao; Chan, Yao Fei; Chen, Yi Chun; Chen, Jyh Yih.

In: Cancers, Vol. 11, No. 1, 107, 01.01.2019.

Research output: Contribution to journalArticle

Ting, Chen Hung ; Lee, Kang Yun ; Wu, Sheng Ming ; Feng, Po Hao ; Chan, Yao Fei ; Chen, Yi Chun ; Chen, Jyh Yih. / FOSB-PCDHB13 axis disrupts the microtubule network in non-small cell lung cancer. In: Cancers. 2019 ; Vol. 11, No. 1.
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