Formation and identification of carcinogenic heterocyclic aromatic amines in boiled pork juice

Huei Lee, Ming Yang Lin, Shu Ch in Chan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Bacterial frameshift mutagens have been found in boiled pork juice. The mutagenic compounds of boiled pork juice were purified and analyzed by HPLC. The mutagenic fractions corresponding to the peaks of the standard mutagens 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), and 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ) were confirmed by comparison of UV and mass spectra. One gram equivalent of original lean ground pork was estimated to contain 4.1 ng of MeIQx, 3.7 ng of IQ, and 1.2 ng of MeIQ, which accounted for 21.0%, 30.4%, and 38.1%, respectively, of the total mutagenicity. The remaining mutagenic fraction might be diMeIQx isomers. This will be further confirmed by mass spectrometry in future studies. The amount of IQ-type mutagens in boiled pork juice was about 4-fold higher than in broiled beef. Furthermore, ww investigated the effects of 20 amino acids, three monosaccharides, and creatinine on mutagen formation when added to the pork juice to elucidate possible precursors leading to the formation of mutagens. Results showed that five amino acids (glutamine, tyrosine, glycine, alanine, and threonine) and two monosaccharides (ribose and glucose) might participate in mutagen formation in boiled prok juice.

Original languageEnglish
Pages (from-to)77-88
Number of pages12
JournalMutation Research Regular Papers
Volume308
Issue number1
DOIs
Publication statusPublished - Jul 1 1994
Externally publishedYes

Fingerprint

2-amino-3,4-dimethylimidazo(4,5-f)quinoline
Mutagens
Amines
2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline
Monosaccharides
2-amino-3-methylimidazo(4,5-f)quinoline
Amino Acids
Ribose
Threonine
Glutamine
Alanine
Glycine
Tyrosine
Red Meat
Mass Spectrometry
Creatinine
High Pressure Liquid Chromatography
Glucose

Keywords

  • Boiled pork juice
  • Heterocyclic aromatic amines

ASJC Scopus subject areas

  • Molecular Biology
  • Health, Toxicology and Mutagenesis

Cite this

Formation and identification of carcinogenic heterocyclic aromatic amines in boiled pork juice. / Lee, Huei; Lin, Ming Yang; Chan, Shu Ch in.

In: Mutation Research Regular Papers, Vol. 308, No. 1, 01.07.1994, p. 77-88.

Research output: Contribution to journalArticle

Lee, Huei ; Lin, Ming Yang ; Chan, Shu Ch in. / Formation and identification of carcinogenic heterocyclic aromatic amines in boiled pork juice. In: Mutation Research Regular Papers. 1994 ; Vol. 308, No. 1. pp. 77-88.
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AB - Bacterial frameshift mutagens have been found in boiled pork juice. The mutagenic compounds of boiled pork juice were purified and analyzed by HPLC. The mutagenic fractions corresponding to the peaks of the standard mutagens 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), and 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ) were confirmed by comparison of UV and mass spectra. One gram equivalent of original lean ground pork was estimated to contain 4.1 ng of MeIQx, 3.7 ng of IQ, and 1.2 ng of MeIQ, which accounted for 21.0%, 30.4%, and 38.1%, respectively, of the total mutagenicity. The remaining mutagenic fraction might be diMeIQx isomers. This will be further confirmed by mass spectrometry in future studies. The amount of IQ-type mutagens in boiled pork juice was about 4-fold higher than in broiled beef. Furthermore, ww investigated the effects of 20 amino acids, three monosaccharides, and creatinine on mutagen formation when added to the pork juice to elucidate possible precursors leading to the formation of mutagens. Results showed that five amino acids (glutamine, tyrosine, glycine, alanine, and threonine) and two monosaccharides (ribose and glucose) might participate in mutagen formation in boiled prok juice.

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