Follow-up in the micronucleus frequencies and its subsets in human population with chronic low-dose γ-irradiation exposure

Wushou P. Chang, Mon Shiung Tsai, Jing Shiang Hwang, Yi Ping Lin, Wan Hwa A Hsieh, Huang Shao-Yi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Forty-eight individuals, who received protracted low-dose rate γ-irradiation from radioactive environments for 2-10 years, have been evaluated repetitively for cytogenetic damage by the cytochalasin-B micronuclei assay (CBMN) after they relocated from radioactive buildings. These subjects were shown to have a significant decrease in the CBMN frequencies during 26.2±8.4 months of follow-up. By the mixed effect multiple linear regression analysis, the CBMN frequencies in these 48 subjects during repetitive measurements were significantly associated with the relocation duration since leaving the radioactive environments (relocation time or RT in months; estimate -0.47, standard error 0.0016, p value 0.0074). The alteration rate in the proportions of binucleates carrying a single micronucleus and those with multiple micronuclei was further compared among 26 of these exposed individuals. The proportions of binucleates with multi-micronuclei were shown to decline significantly faster than those with a mono-micronucleus between these two repetitive assays (proportional Z-test, p value 0.003). Moreover, some of the exposed subjects were shown to have a persistent increase in the total micronuclei frequencies or carrying multi-micronuclei in the binuclei even 3-4 years post-cessation of exposure. This suggests potential genomic instability in stem cells of the exposed individuals and the phenomenon deserves further closer monitoring. Understanding the dynamics of micronucleus expression in lymphocytes in subjects with previous mutagenic exposure would be of significant importance for human population monitoring. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)99-105
Number of pages7
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume428
Issue number1-2
DOIs
Publication statusPublished - Jul 16 1999
Externally publishedYes

Fingerprint

Micronucleus Tests
Cytochalasin B
Population
Genomic Instability
Cytogenetics
Linear Models
Stem Cells
Regression Analysis
Lymphocytes

Keywords

  • γ-Irradiation
  • Human lymphocyte
  • Micronucleus frequency
  • Multi
  • Single

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Molecular Biology

Cite this

Follow-up in the micronucleus frequencies and its subsets in human population with chronic low-dose γ-irradiation exposure. / Chang, Wushou P.; Tsai, Mon Shiung; Hwang, Jing Shiang; Lin, Yi Ping; Hsieh, Wan Hwa A; Shao-Yi, Huang.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 428, No. 1-2, 16.07.1999, p. 99-105.

Research output: Contribution to journalArticle

Chang, Wushou P. ; Tsai, Mon Shiung ; Hwang, Jing Shiang ; Lin, Yi Ping ; Hsieh, Wan Hwa A ; Shao-Yi, Huang. / Follow-up in the micronucleus frequencies and its subsets in human population with chronic low-dose γ-irradiation exposure. In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 1999 ; Vol. 428, No. 1-2. pp. 99-105.
@article{e35ad91a5e284a19b6630132dc86e19d,
title = "Follow-up in the micronucleus frequencies and its subsets in human population with chronic low-dose γ-irradiation exposure",
abstract = "Forty-eight individuals, who received protracted low-dose rate γ-irradiation from radioactive environments for 2-10 years, have been evaluated repetitively for cytogenetic damage by the cytochalasin-B micronuclei assay (CBMN) after they relocated from radioactive buildings. These subjects were shown to have a significant decrease in the CBMN frequencies during 26.2±8.4 months of follow-up. By the mixed effect multiple linear regression analysis, the CBMN frequencies in these 48 subjects during repetitive measurements were significantly associated with the relocation duration since leaving the radioactive environments (relocation time or RT in months; estimate -0.47, standard error 0.0016, p value 0.0074). The alteration rate in the proportions of binucleates carrying a single micronucleus and those with multiple micronuclei was further compared among 26 of these exposed individuals. The proportions of binucleates with multi-micronuclei were shown to decline significantly faster than those with a mono-micronucleus between these two repetitive assays (proportional Z-test, p value 0.003). Moreover, some of the exposed subjects were shown to have a persistent increase in the total micronuclei frequencies or carrying multi-micronuclei in the binuclei even 3-4 years post-cessation of exposure. This suggests potential genomic instability in stem cells of the exposed individuals and the phenomenon deserves further closer monitoring. Understanding the dynamics of micronucleus expression in lymphocytes in subjects with previous mutagenic exposure would be of significant importance for human population monitoring. Copyright (C) 1999 Elsevier Science B.V.",
keywords = "γ-Irradiation, Human lymphocyte, Micronucleus frequency, Multi, Single",
author = "Chang, {Wushou P.} and Tsai, {Mon Shiung} and Hwang, {Jing Shiang} and Lin, {Yi Ping} and Hsieh, {Wan Hwa A} and Huang Shao-Yi",
year = "1999",
month = "7",
day = "16",
doi = "10.1016/S1383-5742(99)00036-8",
language = "English",
volume = "428",
pages = "99--105",
journal = "Mutation Research",
issn = "0027-5107",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Follow-up in the micronucleus frequencies and its subsets in human population with chronic low-dose γ-irradiation exposure

AU - Chang, Wushou P.

AU - Tsai, Mon Shiung

AU - Hwang, Jing Shiang

AU - Lin, Yi Ping

AU - Hsieh, Wan Hwa A

AU - Shao-Yi, Huang

PY - 1999/7/16

Y1 - 1999/7/16

N2 - Forty-eight individuals, who received protracted low-dose rate γ-irradiation from radioactive environments for 2-10 years, have been evaluated repetitively for cytogenetic damage by the cytochalasin-B micronuclei assay (CBMN) after they relocated from radioactive buildings. These subjects were shown to have a significant decrease in the CBMN frequencies during 26.2±8.4 months of follow-up. By the mixed effect multiple linear regression analysis, the CBMN frequencies in these 48 subjects during repetitive measurements were significantly associated with the relocation duration since leaving the radioactive environments (relocation time or RT in months; estimate -0.47, standard error 0.0016, p value 0.0074). The alteration rate in the proportions of binucleates carrying a single micronucleus and those with multiple micronuclei was further compared among 26 of these exposed individuals. The proportions of binucleates with multi-micronuclei were shown to decline significantly faster than those with a mono-micronucleus between these two repetitive assays (proportional Z-test, p value 0.003). Moreover, some of the exposed subjects were shown to have a persistent increase in the total micronuclei frequencies or carrying multi-micronuclei in the binuclei even 3-4 years post-cessation of exposure. This suggests potential genomic instability in stem cells of the exposed individuals and the phenomenon deserves further closer monitoring. Understanding the dynamics of micronucleus expression in lymphocytes in subjects with previous mutagenic exposure would be of significant importance for human population monitoring. Copyright (C) 1999 Elsevier Science B.V.

AB - Forty-eight individuals, who received protracted low-dose rate γ-irradiation from radioactive environments for 2-10 years, have been evaluated repetitively for cytogenetic damage by the cytochalasin-B micronuclei assay (CBMN) after they relocated from radioactive buildings. These subjects were shown to have a significant decrease in the CBMN frequencies during 26.2±8.4 months of follow-up. By the mixed effect multiple linear regression analysis, the CBMN frequencies in these 48 subjects during repetitive measurements were significantly associated with the relocation duration since leaving the radioactive environments (relocation time or RT in months; estimate -0.47, standard error 0.0016, p value 0.0074). The alteration rate in the proportions of binucleates carrying a single micronucleus and those with multiple micronuclei was further compared among 26 of these exposed individuals. The proportions of binucleates with multi-micronuclei were shown to decline significantly faster than those with a mono-micronucleus between these two repetitive assays (proportional Z-test, p value 0.003). Moreover, some of the exposed subjects were shown to have a persistent increase in the total micronuclei frequencies or carrying multi-micronuclei in the binuclei even 3-4 years post-cessation of exposure. This suggests potential genomic instability in stem cells of the exposed individuals and the phenomenon deserves further closer monitoring. Understanding the dynamics of micronucleus expression in lymphocytes in subjects with previous mutagenic exposure would be of significant importance for human population monitoring. Copyright (C) 1999 Elsevier Science B.V.

KW - γ-Irradiation

KW - Human lymphocyte

KW - Micronucleus frequency

KW - Multi

KW - Single

UR - http://www.scopus.com/inward/record.url?scp=0032785388&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032785388&partnerID=8YFLogxK

U2 - 10.1016/S1383-5742(99)00036-8

DO - 10.1016/S1383-5742(99)00036-8

M3 - Article

C2 - 10517983

AN - SCOPUS:0032785388

VL - 428

SP - 99

EP - 105

JO - Mutation Research

JF - Mutation Research

SN - 0027-5107

IS - 1-2

ER -